| Autor: |
Annunziata L; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', Via Campo Boario, 64100, Teramo, Italy., Stramenga A; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via G. Salvemini 1, 06126, Perugia, Italy., Visciano P; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini 1, 64100, Teramo, Italy. pvisciano@unite.it., Schirone M; Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via R. Balzarini 1, 64100, Teramo, Italy., De Colli L; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', Via Campo Boario, 64100, Teramo, Italy., Colagrande MN; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', Via Campo Boario, 64100, Teramo, Italy., Campana G; Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', Via Campo Boario, 64100, Teramo, Italy., Scortichini G; Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Via G. Salvemini 1, 06126, Perugia, Italy. |
| Abstrakt: |
A fast, easy, and cheap method for the simultaneous determination and quantification of aflatoxins (B1, B2, G1, G2), T-2 and HT-2 toxins, and fumonisins (B1, B2) in cereal-derived products was developed. This method involved a quick, easy, cheap, effective, rugged, and safe (QuEChERS) extraction coupled with liquid chromatography-tandem mass spectrometry. The method validation was performed by analyzing samples spiked at four levels, and the recoveries ranged from 83.6 to 102.9%, whereas the maximum values of repeatability and within-laboratory reproducibility were 14.3 and 15.7% following the performance criteria set by the European legislation. The method was then applied for the analysis of 21 cereal-derived products purchased on the Italian market, which were correctly packaged and labeled as intended for human consumption. The co-occurrence of more than one mycotoxin in the analyzed samples could represent a risk for consumers, and the described method could be a valid alternative for their simultaneous detection in the framework of official control. Graphical Abstract ᅟ. |
