Collection, processing and freezing of equine bone marrow cells.

Autor: Bastos FZ; Department of Animal Science, School of Life Sciences - Pontifícia Universidade Católica Do Paraná, Curitiba, Paraná, Brazil., Barussi FCM; Department of Animal Science, School of Life Sciences - Pontifícia Universidade Católica Do Paraná, Curitiba, Paraná, Brazil., Santi TF; Course of Veterinary Medicine, School of Life Sciences - Pontifícia Universidade Católica Do Paraná, Curitiba, Paraná, Brazil., Vieira BP; Course of Veterinary Medicine, School of Life Sciences - Pontifícia Universidade Católica Do Paraná, Curitiba, Paraná, Brazil., Senegaglia AC; Experimental Laboratory for Cell Culture, School of Medicine- Pontifícia Universidade Católica Do Paraná, Curitiba, Paraná, Brazil., Cruz FF; Laboratory of Pulmonary Investigation, Carlos Chagas Filho Biophysics Institute, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil., Michelotto PV Jr; Department of Animal Science, School of Life Sciences - Pontifícia Universidade Católica Do Paraná, Curitiba, Paraná, Brazil. Electronic address: p.michelotto@pucpr.br.
Jazyk: angličtina
Zdroj: Cryobiology [Cryobiology] 2017 Oct; Vol. 78, pp. 95-100. Date of Electronic Publication: 2017 Jun 20.
DOI: 10.1016/j.cryobiol.2017.06.006
Abstrakt: There is no consensus on aspects of equine bone marrow collection and processing. The study aimed to describe the collection of large volumes of bone marrow from horses of advanced age, with emphasis on bone marrow mononuclear cells (BMMCs) recovery and viability after cryopreservation. Fourteen horses, aged 3-24 years, were divided into three experiments. E1 studied the feasibility of collecting 200 mL from the sternums of horses of advanced age; E2 examined the number of cells obtained from the first and last syringe of each puncture; and E3 investigated the influence of heparin concentration on the prevention of cell aggregation, and cell viability after freezing in liquid nitrogen. Bone marrow aspirations were done with syringes pre-filled with Iscove's modified Dulbecco's medium and different concentrations of sodium heparin. BMMCs were counted, cell viability was determined, and samples were frozen. Bone marrow collection from the sternum is safe, even at large volumes and from horses of advanced age, and the number of cells recovered decreases with successive aspirations (p < 0.0001). Heparin concentration influenced cell aggregation, and recovered cells continued to be commercially viable after 150 days in frozen storage.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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