Dendrimer-Based Signal Amplification of Click-Labelled DNA in Situ.

Autor: Raddaoui N; Center for Integrated Protein Science (CiPSM), Department of Chemistry, LMU München, Butenandtstrasse 5-13, 81377, München, Germany., Stazzoni S; Center for Integrated Protein Science (CiPSM), Department of Chemistry, LMU München, Butenandtstrasse 5-13, 81377, München, Germany., Möckl L; Center for Integrated Protein Science (CiPSM), Department of Chemistry, LMU München, Butenandtstrasse 5-13, 81377, München, Germany., Viverge B; Center for Integrated Protein Science (CiPSM), Department of Chemistry, LMU München, Butenandtstrasse 5-13, 81377, München, Germany., Geiger F; Center for Integrated Protein Science (CiPSM), Department of Chemistry, LMU München, Butenandtstrasse 5-13, 81377, München, Germany., Engelke H; Center for Integrated Protein Science (CiPSM), Department of Chemistry, LMU München, Butenandtstrasse 5-13, 81377, München, Germany., Bräuchle C; Center for Integrated Protein Science (CiPSM), Department of Chemistry, LMU München, Butenandtstrasse 5-13, 81377, München, Germany., Carell T; Center for Integrated Protein Science (CiPSM), Department of Chemistry, LMU München, Butenandtstrasse 5-13, 81377, München, Germany.
Jazyk: angličtina
Zdroj: Chembiochem : a European journal of chemical biology [Chembiochem] 2017 Sep 05; Vol. 18 (17), pp. 1716-1720. Date of Electronic Publication: 2017 Jul 24.
DOI: 10.1002/cbic.201700209
Abstrakt: The in vivo incorporation of alkyne-modified bases into the genome of cells is today the basis for the efficient detection of cell proliferation. Cells are grown in the presence of ethinyl-dU (EdU), fixed and permeabilised. The incorporated alkynes are then efficiently detected by using azide-containing fluorophores and the Cu I -catalysed alkyne-azide click reaction. For a world in which constant improvement in the sensitivity of a given method is driving diagnostic advancement, we developed azide- and alkyne-modified dendrimers that allow the establishment of sandwich-type detection assays that show significantly improved signal intensities and signal-to-noise ratios far beyond that which is currently possible.
(© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
Databáze: MEDLINE
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