| Autor: |
Pie MR; Departamento de Zoologia, Universidade Federal do Paraná, Av. Cel. Francisco H. dos Santos, 100, Jardim das Américas, 81531-980 Curitiba, PR, Brazil., Ströher PR; Departamento de Zoologia, Universidade Federal do Paraná, Av. Cel. Francisco H. dos Santos, 100, Jardim das Américas, 81531-980 Curitiba, PR, Brazil., Agostinis AO; Departamento de Zoologia, Universidade Federal do Paraná, Av. Cel. Francisco H. dos Santos, 100, Jardim das Américas, 81531-980 Curitiba, PR, Brazil., Belmonte-Lopes R; Departamento de Zoologia, Universidade Federal do Paraná, Av. Cel. Francisco H. dos Santos, 100, Jardim das Américas, 81531-980 Curitiba, PR, Brazil., Tadra-Sfeir MZ; Departamento de Bioquímica, Universidade Federal do Paraná, Av. Cel. Francisco H. dos Santos, 100, Jardim das Américas, 81531-980 Curitiba, PR, Brazil., Ostrensky A; Departamento de Zootecnia, Universidade Federal do Paraná, Rua dos Funcionários, 1540, Juvevê, 80035-050 Curitiba, PR, Brazil. |
| Abstrakt: |
The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of the golden mussel in its introduced range. |
