| Autor: |
Gao G; School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, 122 Luoshi Rd., Wuhan, 430070, China.; Stemorgan Therapeutics, 90 State St., Albany, NY, 12207, USA., Hubbell K; Stemorgan Therapeutics, 90 State St., Albany, NY, 12207, USA., Schilling AF; Clinic for Plastic Surgery and Hand Surgery, Klinikum Rechts der Isar, Technical University of Munich, Munich, Germany., Dai G; Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th St., Troy, NY, 12180, USA., Cui X; School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, 122 Luoshi Rd., Wuhan, 430070, China. xfc.cui@gmail.com.; Stemorgan Therapeutics, 90 State St., Albany, NY, 12207, USA. xfc.cui@gmail.com.; Clinic for Plastic Surgery and Hand Surgery, Klinikum Rechts der Isar, Technical University of Munich, Munich, Germany. xfc.cui@gmail.com.; Department of Biomedical Engineering, Rensselaer Polytechnic Institute, 110 8th St., Troy, NY, 12180, USA. xfc.cui@gmail.com. |
| Abstrakt: |
Bioprinting based on thermal inkjet printing is one of the most attractive enabling technologies for tissue engineering and regeneration. During the printing process, cells, scaffolds , and growth factors are rapidly deposited to the desired two-dimensional (2D) and three-dimensional (3D) locations. Ideally, the bioprinted tissues are able to mimic the native anatomic structures in order to restore the biological functions. In this study, a bioprinting platform for 3D cartilage tissue engineering was developed using a commercially available thermal inkjet printer with simultaneous photopolymerization . The engineered cartilage demonstrated native zonal organization, ideal extracellular matrix (ECM ) composition, and proper mechanical properties. Compared to the conventional tissue fabrication approach, which requires extended UV exposure, the viability of the printed cells with simultaneous photopolymerization was significantly higher. Printed neocartilage demonstrated excellent glycosaminoglycan (GAG) and collagen type II production, which was consistent with gene expression profile. Therefore, this platform is ideal for anatomic tissue engineering with accurate cell distribution and arrangement. |
