Association between Plasmatic Ceramides Profile and AST/ALT Ratio: C14:0 Ceramide as Predictor of Hepatic Steatosis in Adolescents Independently of Obesity.

Autor: Maldonado-Hernández J; Laboratorio de Espectrometría de Masas, Unidad de Investigación Médica en Nutrición, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, Mexico., Saldaña-Dávila GE; Laboratorio de Espectrometría de Masas, Unidad de Investigación Médica en Nutrición, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, Mexico., Piña-Aguero MI; Laboratorio de Espectrometría de Masas, Unidad de Investigación Médica en Nutrición, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, Mexico., Núñez-García BA; Laboratorio de Espectrometría de Masas, Unidad de Investigación Médica en Nutrición, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, Mexico., López-Alarcón MG; Laboratorio de Espectrometría de Masas, Unidad de Investigación Médica en Nutrición, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, IMSS, Ciudad de México, Mexico.
Jazyk: angličtina
Zdroj: Canadian journal of gastroenterology & hepatology [Can J Gastroenterol Hepatol] 2017; Vol. 2017, pp. 3689375. Date of Electronic Publication: 2017 May 28.
DOI: 10.1155/2017/3689375
Abstrakt: Objective: To assess the association between plasma ceramides and hepatic steatosis (HS) in adolescents, independently of obesity.
Materials and Methods: Ninety-four adolescents from two previous studies conducted and published by our crew were included. Study subjects were stratified in three groups: normal weight ( n = 18), obesity ( n = 34), and obesity + HS ( n = 42). The presence of HS was defined when ALT/AST ratio was <1. Ceramides subspecies (C14:0, C16:0, C18:0, C24:0, and C24:1) were determined by LC/MS.
Results: All ceramides correlated directly with ALT levels and inversely with ALT/AST ratio; the strongest correlation was observed among C14:0 ceramide ( r = 0.41 and r = -0.54, resp.; P < 0.001). Furthermore, significant correlations were observed between cholesterol and all ceramides except for C24:1 ceramide. Interestingly ceramides C14:0, C18:0, and C24:1 correlated directly with both fasting insulin and HOMA-IR index. For assessing HS, a cut-off point of 10.3 nmol/L for C14:0 ceramide reported a sensitivity of 92.7% and a specificity of 73.5% when normal weight and obesity groups ( n = 52) were compared against obesity + HS group ( n = 42). Positive and negative predictive values were 77.5% and 90.2%, respectively.
Conclusions: Plasma ceramides are closely associated with hepatic steatosis in adolescents. C14:0 ceramide could be a novel biomarker of HS independently of obesity.
Databáze: MEDLINE
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