The Pseudomonas aeruginosa PSL Polysaccharide Is a Social but Noncheatable Trait in Biofilms.

Autor:	Irie Y; School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom yirie1@udayton.edu stephen.diggle@biosci.gatech.edu.; Department of Biology and Biochemistry, University of Bath, Bath, United Kingdom.; Department of Biology, University of Dayton, Dayton, Ohio, USA., Roberts AEL; School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom., Kragh KN; Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark., Gordon VD; Center for Nonlinear Dynamics and Department of Physics, The University of Texas at Austin, Austin, Texas, USA., Hutchison J; Center for Nonlinear Dynamics and Department of Physics, The University of Texas at Austin, Austin, Texas, USA., Allen RJ; School of Physics and Astronomy, University of Edinburgh, Edinburgh, United Kingdom., Melaugh G; Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark., Bjarnsholt T; Department of International Health, Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark.; Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark., West SA; Department of Zoology, University of Oxford, Oxford, United Kingdom., Diggle SP; School of Life Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, United Kingdom yirie1@udayton.edu stephen.diggle@biosci.gatech.edu.
Jazyk:	angličtina
Zdroj:	MBio [mBio] 2017 Jun 20; Vol. 8 (3). Date of Electronic Publication: 2017 Jun 20.
DOI:	10.1128/mBio.00374-17
Abstrakt:	Extracellular polysaccharides are compounds secreted by microorganisms into the surrounding environment, and they are important for surface attachment and maintaining structural integrity within biofilms. The social nature of many extracellular polysaccharides remains unclear, and it has been suggested that they could function as either cooperative public goods or as traits that provide a competitive advantage. Here, we empirically tested the cooperative nature of the PSL polysaccharide, which is crucial for the formation of biofilms in Pseudomonas aeruginosa We show that (i) PSL is not metabolically costly to produce; (ii) PSL provides population-level benefits in biofilms, for both growth and antibiotic tolerance; (iii) the benefits of PSL production are social and are shared with other cells; (iv) the benefits of PSL production appear to be preferentially directed toward cells which produce PSL; (v) cells which do not produce PSL are unable to successfully exploit cells which produce PSL. Taken together, this suggests that PSL is a social but relatively nonexploitable trait and that growth within biofilms selects for PSL-producing strains, even when multiple strains are on a patch (low relatedness at the patch level). IMPORTANCE Many studies have shown that bacterial traits, such as siderophores and quorum sensing, are social in nature. This has led to an impression that secreted traits act as public goods, which are costly to produce but benefit both the producing cell and its surrounding neighbors. Theories and subsequent experiments have shown that such traits are exploitable by asocial cheats, but we show here that this does not always hold true. We demonstrate that the Pseudomonas aeruginosa exopolysaccharide PSL provides social benefits to populations but that it is nonexploitable, because most of the fitness benefits accrue to PSL-producing cells. Our work builds on an increasing body of work showing that secreted traits can have both private and public benefits to cells. (Copyright © 2017 Irie et al.)
Databáze:	MEDLINE
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