| Autor: |
An DS; Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea., Cui CH; KAIST Institute for Biocentury, Korea Advanced Institute of Science and Technology, Daejeon 34141, Republic of Korea., Siddiqi MZ; Department of Biotechnology, Hankyong National University, Anseong 17579, Republic of Korea.; Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyong National University, Anseong 17579, Republic of Korea., Yu HS; College of Biotechnology, Dalian Polytechnic University, Dalian 116034, P.R. China.; University of Science and Technology (UST), Daejeon 34113, Republic of Korea., Jin FX; College of Biotechnology, Dalian Polytechnic University, Dalian 116034, P.R. China.; University of Science and Technology (UST), Daejeon 34113, Republic of Korea., Kim SG; Biological Resource Center/Korean Collection for Type Cultures (KCTC), Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea., Im WT; Department of Biotechnology, Hankyong National University, Anseong 17579, Republic of Korea.; Center for Genetic Information, Graduate School of Bio and Information Technology, Hankyong National University, Anseong 17579, Republic of Korea. |
| Abstrakt: |
Naturally occurring ginsenoside F1 (20-O-β-D-glucopyranosyl-20(S)-protopanaxatriol) is rare. Here, we produced gram-scale quantities of ginsenoside F1 from a crude protopanaxatriol saponin mixture comprised mainly of Re and Rg1 through enzyme-mediated biotransformation using recombinant β-glucosidase (BgpA) cloned from a soil bacterium, Terrabacter ginsenosidimutans Gsoil 3082 T . In a systematic step-by-step process, the concentrations of substrate, enzyme, and NaCl were determined for maximal production of F1. At an optimized NaCl concentration of 200 mM, the protopanaxatriol saponin mixture (25 mg/ml) was incubated with recombinant BgpA (20 mg/ml) for 3 days in a 2.4 L reaction. Following octadecylsilyl silica gel column chromatography, 9.6 g of F1 was obtained from 60 g of substrate mixture at 95% purity, as assessed by chromatography. These results represent the first report of gramscale F1 production via recombinant enzyme-mediated biotransformation. |
