Polyphenol profile by UHPLC-MS/MS, anti-glycation, antioxidant and cytotoxic activities of several samples of propolis from the northeastern semi-arid region of Brazil.

Autor: Xavier JA; a Instituto de Química e Biotecnologia , Universidade Federal de Alagoas (UFAL) , Maceió , AL , Brazil., Valentim IB; b Instituto Federal de Educação , Ciência e Tecnologia de Alagoas (IFAL), Rua Mizael Domingues , Maceió , AL , Brazil., Camatari FOS; a Instituto de Química e Biotecnologia , Universidade Federal de Alagoas (UFAL) , Maceió , AL , Brazil., de Almeida AMM; c Empresa Baiana de Desenvolvimento Agrícola S.A (EBDA) , Salvador , BA , Brazil., Goulart HF; d Laboratório de Pesquisas em Recursos Naturais, Centro de Ciências Agrárias (CECA), UFAL , Rio Largo , AL , Brazil., Ferro JNS; e Laboratório de Biologia Celular, UFAL , Maceio , AL , Brazil., Barreto EO; e Laboratório de Biologia Celular, UFAL , Maceio , AL , Brazil., Cavalcanti BC; f Departamento de Fisiologia e Farmacologia , Laboratório Nacional de Oncologia Experimental, Universidade Federal do Ceará , Fortaleza , CE , Brazil., Bottoli CBG; g Instituto de Química , Universidade de Campinas , Campinas , SP , Brazil., Goulart MOF; a Instituto de Química e Biotecnologia , Universidade Federal de Alagoas (UFAL) , Maceió , AL , Brazil.
Jazyk: angličtina
Zdroj: Pharmaceutical biology [Pharm Biol] 2017 Dec; Vol. 55 (1), pp. 1884-1893.
DOI: 10.1080/13880209.2017.1340962
Abstrakt: Context: Propolis has promising biological activities. Propolis samples from the Northeast of Bahia, Brazil - sample A from Ribeira do Pombal and B, from Tucano - were investigated, with new information regarding their biological activities.
Objective: This paper describes the chemical profile, antioxidant, anti-glycation and cytotoxic activities of these propolis samples.
Material and Methods: Ethanol extracts of these propolis samples (EEP) and their fractions were analyzed to determine total phenolic content (TPC); antioxidant capacity through DPPH • , FRAP and lipid peroxidation; anti-glycation activity, by an in vitro glucose (10 mg/mL) bovine serum albumine (1 mg/mL) assay, during 7 d; cytotoxic activity on cancer (SF295, HCT-116, OVCAR-8, MDA-MB435, MX-1, MCF7, HL60, JURKAT, MOLT-4, K562, PC3, DU145) and normal cell lines (V79) at 0.04-25 μg/mL concentrations, for 72 h. The determination of primary phenols by ultra high-pressure liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) and volatile organic compounds content by gas chromatography-mass spectrometry (GC-MS) were also performed.
Results: The EEP polar fractions exhibited up to 90% protection against lipid peroxidation. The IC 50 value for anti-glycation activity of EEP was between 16.5 and 19.2 μg/mL, close to aminoguanidine (IC 50  = 7.7 μg/mL). The use of UHPLC-MS/MS and GC-MS allowed the identification of 12 bioactive phenols in the EEP and 24 volatile compounds, all already reported.
Conclusions: The samples present good antioxidant/anti-glycation/cytotoxic activities and a plethora of biologically active compounds. These results suggest a potential role of propolis in targeting ageing and diseases associated with oxidative and carbonylic stress, aggregating value to them.
Databáze: MEDLINE
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