Adaptation of tick-borne encephalitis virus from human brain to different cell cultures induces multiple genomic substitutions.

Autor: Ponomareva EP; Department of Molecular Virology for Flaviviruses and Viral Hepatitis, State Research Center for Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk region, 630559, Russia., Ternovoi VA; Department of Molecular Virology for Flaviviruses and Viral Hepatitis, State Research Center for Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk region, 630559, Russia.; Tomsk State University, Tomsk, 634050, Russia., Mikryukova TP; Department of Molecular Virology for Flaviviruses and Viral Hepatitis, State Research Center for Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk region, 630559, Russia.; Tomsk State University, Tomsk, 634050, Russia., Protopopova EV; Department of Molecular Virology for Flaviviruses and Viral Hepatitis, State Research Center for Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk region, 630559, Russia.; Tomsk State University, Tomsk, 634050, Russia., Gladysheva AV; Department of Molecular Virology for Flaviviruses and Viral Hepatitis, State Research Center for Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk region, 630559, Russia., Shvalov AN; Department of Molecular Virology for Flaviviruses and Viral Hepatitis, State Research Center for Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk region, 630559, Russia., Konovalova SN; Department of Molecular Virology for Flaviviruses and Viral Hepatitis, State Research Center for Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk region, 630559, Russia., Chausov EV; Department of Molecular Virology for Flaviviruses and Viral Hepatitis, State Research Center for Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk region, 630559, Russia., Loktev VB; Department of Molecular Virology for Flaviviruses and Viral Hepatitis, State Research Center for Virology and Biotechnology 'Vector', Koltsovo, Novosibirsk region, 630559, Russia. valeryloktev@gmail.com.; Tomsk State University, Tomsk, 634050, Russia. valeryloktev@gmail.com.; Institute of Cytology and Genetics, Novosibirsk, 630090, Russia. valeryloktev@gmail.com.
Jazyk: angličtina
Zdroj: Archives of virology [Arch Virol] 2017 Oct; Vol. 162 (10), pp. 3151-3156. Date of Electronic Publication: 2017 Jun 19.
DOI: 10.1007/s00705-017-3442-x
Abstrakt: The C11-13 strain from the Siberian subtype of tick-borne encephalitis virus (TBEV) was isolated from human brain using pig embryo kidney (PEK), 293, and Neuro-2a cells. Analysis of the complete viral genome of the C11-13 variants during six passages in these cells revealed that the cell-adapted C11-13 variants had multiple amino acid substitutions as compared to TBEV from human brain. Seven out of eight amino acids substitutions in the high-replicating C11-13(PEK) variant mapped to non-structural proteins; 13 out of 14 substitutions in the well-replicating C11-13(293) variant, and all four substitutions in the low-replicating C11-13(Neuro-2a) variant were also localized in non-structural proteins, predominantly in the NS2a (2), NS3 (6) and NS5 (3) proteins. The substitutions NS2a 1067 (Asn → Asp), NS2a 1168 (Leu → Val) in the N-terminus of NS2a and NS3 1745 (His → Gln) in the helicase domain of NS3 were found in all selected variants. We postulate that multiple substitutions in the NS2a, NS3 and NS5 genes play a key role in adaptation of TBEV to different cells.
Databáze: MEDLINE
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