Photodynamic effect of light-harvesting, long-lived triplet excited state Ruthenium(II)-polyimine-coumarin complexes: DNA binding, photocleavage and anticancer studies.

Autor: Nomula R; Centre for Materials for Electronics Technology (C-MET), IDA Phase-III, Cherlapally, HCL Post, Hyderabad 500051, India., Wu X; State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, PR China., Zhao J; State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, PR China. Electronic address: zhaojzh@dlut.edu.cn., Munirathnam NR; Centre for Materials for Electronics Technology (C-MET), IDA Phase-III, Cherlapally, HCL Post, Hyderabad 500051, India. Electronic address: rathnam@cmet.gov.in.
Jazyk: angličtina
Zdroj: Materials science & engineering. C, Materials for biological applications [Mater Sci Eng C Mater Biol Appl] 2017 Oct 01; Vol. 79, pp. 710-719. Date of Electronic Publication: 2017 May 17.
DOI: 10.1016/j.msec.2017.05.123
Abstrakt: Two coumarin based Ru II -polyimine complexes (Ru-1 and Ru-2) showing intense absorption of visible light and long-lived triplet excited states (~12-15μs) were used for study of the interaction with DNA. The binding of the complexes with CT-DNA were studied by UV-vis, fluorescence and time-resolved nanosecond transient absorption (ns-TA) spectroscopy. The results suggesting that the complexes interact with CT-DNA by intercalation mode of binding, showing the binding constants (K b ) 6.47×10 4 for Ru-1 and 5.94×10 4 M -1 for Ru-2, in contrast no such results were found for Ru-0. The nanosecond transient absorption spectra of these systems in the presence of CT-DNA showing a clear perturbation in the bleaching region was observed compare to buffer alone. Visible light photoirradiation DNA cleavage was investigated for these complexes by treating with the supercoiled pUC19 DNA and irradiated at 450nm. The reactive species produced upon irradiation of current agents is singlet oxygen ( 1 O 2 ), which results in the generation of other reactive oxygen species (ROS). The complexes shown efficient cleavage activity, converted complete supercoiled DNA to nicked circular at as low as 20μM concentration in 30min of light irradiation time. Significant amount of linear form was generated by Ru-1 at the same conditions. Even though Ru-0 has significant 1 O 2 quantum yield but shown lower cleavage activity compared to other two analogs is due the miserable interaction (binding) with DNA. The cytotoxicity in vitro of the complexes toward HeLa, BEL-7402 and MG-63 cells was assessed by MTT assay. The cellular uptake was observed on BEL-7402 cells under fluorescence microscope. The complexes shown appreciable cytotoxicity towards the cancer cell lines.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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