Progress in programming spatiotemporal patterns and machine-assembly in cell-free protein expression systems.

Autor: Tayar AM; Department of Materials and Interfaces, The Weizmann Institute of Science, Rehovot 76100, Israel., Daube SS; Department of Materials and Interfaces, The Weizmann Institute of Science, Rehovot 76100, Israel., Bar-Ziv RH; Department of Materials and Interfaces, The Weizmann Institute of Science, Rehovot 76100, Israel. Electronic address: roy.bar-ziv@weizmann.ac.il.
Jazyk: angličtina
Zdroj: Current opinion in chemical biology [Curr Opin Chem Biol] 2017 Oct; Vol. 40, pp. 37-46. Date of Electronic Publication: 2017 Jun 16.
DOI: 10.1016/j.cbpa.2017.05.005
Abstrakt: Building biological systems outside the cell is an emerging interdisciplinary research field aimed to study design principles, and to emulate biological functions for technology. Reconstructing programmable cellular functions, from assembly of protein/nucleic-acid machines to spatially distributed systems, requires implementing minimal systems of molecular interactions encoded in genes, source-sink protein expression dynamics, and materials platforms for reaction-diffusion scenarios. Here, we first review how molecular turnover mechanisms, combined with nonlinear interactions and feedback in cell-free gene networks enable programmable dynamic expression patterns in various compartments. We then describe recent work on spatially distributed protein expression reactions. Finally, we discuss progress and challenges in the study of programmable protein/nucleic-acid complexes.
(Copyright © 2017. Published by Elsevier Ltd.)
Databáze: MEDLINE
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