Coagulase-negative staphylococci species affect biofilm formation of other coagulase-negative and coagulase-positive staphylococci.

Autor:	Goetz C; Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, St-Hyacinthe, Québec, Canada J2S 2M2; Canadian Bovine Mastitis and Milk Quality Research Network, St-Hyacinthe, Québec, Canada J2S 2M2., Tremblay YDN; Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, St-Hyacinthe, Québec, Canada J2S 2M2; Canadian Bovine Mastitis and Milk Quality Research Network, St-Hyacinthe, Québec, Canada J2S 2M2., Lamarche D; Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, St-Hyacinthe, Québec, Canada J2S 2M2., Blondeau A; Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, St-Hyacinthe, Québec, Canada J2S 2M2., Gaudreau AM; Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, St-Hyacinthe, Québec, Canada J2S 2M2., Labrie J; Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, St-Hyacinthe, Québec, Canada J2S 2M2., Malouin F; Canadian Bovine Mastitis and Milk Quality Research Network, St-Hyacinthe, Québec, Canada J2S 2M2; Department of Biology, Faculty of Science, Université de Sherbrooke, Sherbrooke, Québec, Canada J1K 2R1., Jacques M; Department of Pathology and Microbiology, Faculty of Veterinary Medicine, Université de Montréal, St-Hyacinthe, Québec, Canada J2S 2M2; Canadian Bovine Mastitis and Milk Quality Research Network, St-Hyacinthe, Québec, Canada J2S 2M2. Electronic address: mario.jacques@umontreal.ca.
Jazyk:	angličtina
Zdroj:	Journal of dairy science [J Dairy Sci] 2017 Aug; Vol. 100 (8), pp. 6454-6464. Date of Electronic Publication: 2017 Jun 16.
DOI:	10.3168/jds.2017-12629
Abstrakt:	Coagulase-negative staphylococci (CNS) are considered to be commensal bacteria in humans and animals, but are now also recognized as etiological agents in several infections, including bovine mastitis. Biofilm formation appears to be an important factor in CNS pathogenicity. Furthermore, some researchers have proposed that CNS colonization of the intramammary environment has a protective effect against other pathogens. The mechanisms behind the protective effect of CNS have yet to be characterized. The aim of this study was to evaluate the effect of CNS isolates with a weak-biofilm phenotype on the biofilm formation of other staphylococcal isolates. We selected 10 CNS with a weak-biofilm phenotype and 30 staphylococcal isolates with a strong-biofilm phenotype for this study. We measured biofilm production by individual isolates using a standard polystyrene microtiter plate assay and compared the findings with biofilm produced in mixed cultures. We confirmed the results using confocal microscopy and a microfluidic system with low shear force. Four of the CNS isolates with a weak-biofilm phenotype (Staphylococcus chromogenes C and E and Staphylococcus simulans F and H) significantly reduced biofilm formation in approximately 80% of the staphylococcal species tested, including coagulase-positive Staphylococcus aureus. The 4 Staph. chromogenes and Staph. simulans isolates were also able to disperse pre-established biofilms, but to a lesser extent. We also performed a deferred antagonism assay and recorded the number of colony-forming units in the mixed-biofilm assays on differential or selective agar plates. Overall, CNS with a weak-biofilm phenotype did not inhibit the growth of isolates with a strong-biofilm phenotype. These results suggest that some CNS isolates can negatively affect the ability of other staphylococcal isolates and species to form biofilms via a mechanism that does not involve growth inhibition. (Copyright © 2017 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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