BCOR is a robust diagnostic immunohistochemical marker of genetically diverse high-grade endometrial stromal sarcoma, including tumors exhibiting variant morphology.

Autor: Chiang S; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Lee CH; Department of Pathology, British Columbia Cancer Agency, Vancouver, BC, Canada.; Department of Pathology, Vancouver General Hospital, Vancouver, BC, Canada., Stewart CJR; Department of Pathology, King Edward Memorial Hospital, Perth, Western Australia, Australia., Oliva E; Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston, MA, USA., Hoang LN; Department of Pathology, Vancouver General Hospital, Vancouver, BC, Canada., Ali RH; Department of Pathology, Kuwait University Health Sciences Center, Kuwait City, Kuwait., Hensley ML; Department of Medicine, Gynecologic Medical Oncology Service, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Arias-Stella JA 3rd; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Frosina D; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Jungbluth AA; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Benayed R; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Ladanyi M; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Hameed M; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Wang L; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Kao YC; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA.; Department of Pathology, Shuang Ho Hospital, Taipei Medical University, Taipei, Taiwan., Antonescu CR; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Soslow RA; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Jazyk: angličtina
Zdroj: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc [Mod Pathol] 2017 Sep; Vol. 30 (9), pp. 1251-1261. Date of Electronic Publication: 2017 Jun 16.
DOI: 10.1038/modpathol.2017.42
Abstrakt: Recognition of high-grade endometrial stromal sarcoma is important because of its aggressive clinical behavior. Morphologic features of YWHAE-NUTM2 high-grade endometrial stromal sarcoma may overlap with other uterine sarcoma types. BCOR immunoexpression was studied in these tumors and their morphologic mimics to assess its diagnostic utility. BCOR immunohistochemical staining was performed on archival tissue from 28 high-grade endometrial stromal sarcomas with classic morphology (20 YWHAE-NUTM2, 5 ZC3H7B-BCOR, 3 BCOR-ZC3H7B), 3 high-grade endometrial stromal sarcomas with unusual morphology and unknown gene rearrangement status, 66 low-grade endometrial stromal sarcomas, 21 endometrial stromal nodules, 38 uterine leiomyosarcomas, and 19 uterine leiomyomas. Intensity of nuclear staining and percentage of positive tumor cells were recorded. Strong diffuse nuclear BCOR staining (defined as >95% of tumor cells) was seen in the round cell component of all 20 (100%) classic YWHAE-NUTM2 high-grade endometrial stromal sarcomas and the 3 unusual high-grade endometrial stromal sarcomas which prompted FISH studies confirming YWHAE rearrangement in 2 tumors. Genomic PCR confirmed the presence of BCOR exon 16 internal tandem duplication in the third case. Diffuse BCOR staining was strong in three and weak in one BCOR-rearranged high-grade endometrial stromal sarcoma while absent in the remaining four BCOR-rearranged tumors. BCOR staining was weakly positive in <5% of tumor cells in 4 of 66 (6%) low-grade endometrial stromal sarcomas and 1 of 18 (6%) endometrial stromal nodules and weakly to moderately positive in <5-40% of tumor cells in 6 of 31 (19%) leiomyosarcomas. No BCOR staining was seen in the remaining low-grade endometrial stromal sarcomas, endometrial stromal nodules, leiomyosarcomas, or any of the leiomyomas. BCOR immunohistochemical staining is a highly sensitive marker for YWHAE-NUTM2 high-grade endometrial stromal sarcoma with both classic and unusual morphology and identifies a subset of high-grade endometrial stromal sarcoma with BCOR alterations, including BCOR rearrangement and internal tandem duplication.
Databáze: MEDLINE
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