The effects of iloprost and alprostadil on ischemia-reperfusion injury in preventing inflammation, tissue degeneration, and apoptosis in rat skeletal muscle.

Autor: Güneysu E; Department of Cardiovascular Surgery, Bakirköy Dr Sadi Konuk Research and Training Hospital, İstanbul, Turkey., Koçman AE; Department of Plastic Reconstructive Aesthetic Surgery, Faculty of Medicine, Eskişehir Osmangazi University, Eskişehir, Turkey., Özatik O; Department of Histology and Embryology, Faculty of Medicine, Ahi Evran University, Kırşehir, Turkey., Ovali C; Department of Cardiovascular Surgery, Faculty of Medicine, Eskişehir Osmangazi University, Eskişehir, Turkey., Can B; Department of Biochemistry, Faculty of Medicine, Eskişehir Osmangazi University, Eskişehir, Turkey., Alataş İÖ; Department of Biochemistry, Faculty of Medicine, Eskişehir Osmangazi University, Eskişehir, Turkey., Sevin MB; Department of Cardiovascular Surgery, Faculty of Medicine, Eskişehir Osmangazi University, Eskişehir, Turkey.
Jazyk: angličtina
Zdroj: Turkish journal of medical sciences [Turk J Med Sci] 2017 Jun 12; Vol. 47 (3), pp. 1028-1036. Date of Electronic Publication: 2017 Jun 12.
DOI: 10.3906/sag-1604-59
Abstrakt: Background/aim: The protective effects of prostaglandin (PG) analogs on ischemia-reperfusion (I/R) have been well documented; however, comparative studies are lacking. The aim of the present study was to determine whether iloprost or alprostadil is more effective in preventing muscle I/R injury.
Materials and Methods: Thirty-two rats were divided into four groups (n = 8): sham, control, IL (I/R + iloprost), and AL (I/R + alprostadil). I/R was induced by a tourniquet in the hindlimb for 3 h/3 h. The IL and AL groups received iloprost (0.5 ng kg -1 min -1 ) and alprostadil (0.05 µg kg -1 min -1 ) during reperfusion, respectively. After 6 h, blood and muscles were collected for analyses.
Results: Serum TNF-α and IL-1β levels were decreased in the IL and AL groups compared with the control group (P < 0.05), whereas IL-6 levels did not change significantly. Tissue malondialdehyde levels were significantly lower in the IL and AL groups (P < 0.05). Tissue catalase levels showed no difference. The histological damage scores and apoptosis scores were both significantly decreased in the IL and AL groups compared with the control group (P< 0.05).
Conclusion: The present study indicated that iloprost and alprostadil attenuated I/R injury in skeletal muscle. However, no comparable difference was evident regarding the efficacies of either PG analog.
Databáze: MEDLINE
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