| Autor: |
Mayer JU; Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Medicine, Veterinary Medicine and Life Sciences, University of Glasgow, 120 University Place, Glasgow G12 8TA, UK., Demiri M; Immunology Section, Lund University, Sölvegatan 19, Lund BMC D14, Sweden., Agace WW; Immunology Section, Lund University, Sölvegatan 19, Lund BMC D14, Sweden.; Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark, Bülowsvej 27, Frederiksberg C 1870, Denmark., MacDonald AS; Faculty of Biology, Medicine and Health, Manchester Collaborative Centre for Inflammation Research, School of Biological Sciences, The University of Manchester, 46 Grafton Street, Manchester M13 9NT, UK., Svensson-Frej M; Immunology Section, Lund University, Sölvegatan 19, Lund BMC D14, Sweden., Milling SW; Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Medicine, Veterinary Medicine and Life Sciences, University of Glasgow, 120 University Place, Glasgow G12 8TA, UK. |
| Abstrakt: |
T-helper 2 (Th2) cell responses defend against parasites. Although dendritic cells (DCs) are vital for the induction of T-cell responses, the DC subpopulations that induce Th2 cells in the intestine are unidentified. Here we show that intestinal Th2 responses against Trichuris muris worms and Schistosoma mansoni eggs do not develop in mice with IRF-4-deficient DCs (IRF-4 f/f CD11c-cre). Adoptive transfer of conventional DCs, in particular CD11b-expressing DCs from the intestine, is sufficient to prime S. mansoni-specific Th2 responses. Surprisingly, transferred IRF-4-deficient DCs also effectively prime S. mansoni-specific Th2 responses. Egg antigens do not induce the expression of IRF-4-related genes. Instead, IRF-4 f/f CD11c-cre mice have fewer CD11b + migrating DCs and fewer DCs carrying parasite antigens to the lymph nodes. Furthermore, CD11b + CD103 + DCs induce Th2 responses in the small intestine, whereas CD11b + CD103 - DCs perform this role in the colon, revealing a specific functional heterogeneity among intestinal DCs in inducing Th2 responses. |
