Warning About the Use of Critical-Size Defects for the Translational Study of Bone Repair: Analysis of a Sheep Tibial Model.

Autor:	Lammens J; 1 Department of Orthopaedic Surgery, University Hospitals KU Leuven , Leuven, Belgium .; 2 Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, Leuven, Belgium ., Maréchal M; 2 Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, Leuven, Belgium ., Geris L; 2 Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, Leuven, Belgium .; 3 Biomechanics Section, Department of Mechanical Engineering, KU Leuven, Leuven, Belgium .; 6 Biomechanics Research Unit, GIGA in Silico Medicine, University of Liège, Liège, Belgium ., Van der Aa J; 4 Faculty of Medicine, KU Leuven, Leuven, Belgium ., Van Hauwermeiren H; 5 Medanex Clinic, Facility for Preclinical CRO, Diest, Belgium ., Luyten FP; 2 Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, Leuven, Belgium ., Delport H; 1 Department of Orthopaedic Surgery, University Hospitals KU Leuven , Leuven, Belgium .; 2 Prometheus, Division of Skeletal Tissue Engineering, KU Leuven, Leuven, Belgium .
Jazyk:	angličtina
Zdroj:	Tissue engineering. Part C, Methods [Tissue Eng Part C Methods] 2017 Nov; Vol. 23 (11), pp. 694-699. Date of Electronic Publication: 2017 Jul 03.
DOI:	10.1089/ten.TEC.2017.0147
Abstrakt:	The repair of large long bone defects requires complex surgical procedures as the bone loss cannot simply be replaced by autologous grafts due to an insufficient bone stock of the human body. Tissue engineering strategies and the use of Advanced Therapy Medicinal Products (ATMPs) for these reconstructions remain a considerable challenge, in particular since robust outcomes in well-defined large animal models are lacking. To be suitable as a model for treatment of human sized bone defects, we developed a large animal model in both skeletally immature and mature sheep and made close observations on the spontaneous healing of defects. We warn for the spontaneous repair of large defects in immature animals, which can mask the (in)effectiveness of ATMP therapies, and propose the use of large 4.5 cm defects that are pretreated with a polymethylmethacrylate (PMMA) spacer in skeletally mature animals.
Databáze:	MEDLINE
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