Computational segmentation of collagen fibers in bone matrix indicates bone quality in ovariectomized rat spine.

Autor: Daghma DES; Experimental Trauma Surgery, Justus-Liebig University, Giessen, Aulweg 128, 35392, Giessen, Germany., Malhan D; Experimental Trauma Surgery, Justus-Liebig University, Giessen, Aulweg 128, 35392, Giessen, Germany., Simon P; Max Planck Institute for Chemical Physics of Solids, Dresden, Germany., Stötzel S; Experimental Trauma Surgery, Justus-Liebig University, Giessen, Aulweg 128, 35392, Giessen, Germany., Kern S; Experimental Trauma Surgery, Justus-Liebig University, Giessen, Aulweg 128, 35392, Giessen, Germany., Hassan F; Experimental Trauma Surgery, Justus-Liebig University, Giessen, Aulweg 128, 35392, Giessen, Germany., Lips KS; Experimental Trauma Surgery, Justus-Liebig University, Giessen, Aulweg 128, 35392, Giessen, Germany., Heiss C; Experimental Trauma Surgery, Justus-Liebig University, Giessen, Aulweg 128, 35392, Giessen, Germany.; Department of Trauma, Hand and Reconstructive Surgery, University Hospital of Giessen-Marburg, Campus Giessen, Giessen, Germany., El Khassawna T; Experimental Trauma Surgery, Justus-Liebig University, Giessen, Aulweg 128, 35392, Giessen, Germany. Thaqif.elkhassawna@chiru.med.uni-giessen.de.
Jazyk: angličtina
Zdroj: Journal of bone and mineral metabolism [J Bone Miner Metab] 2018 May; Vol. 36 (3), pp. 297-306. Date of Electronic Publication: 2017 Jun 06.
DOI: 10.1007/s00774-017-0844-5
Abstrakt: Bone loss varies according to disease and age and these variations affect bone cells and extracellular matrix. Osteoporosis rat models are widely investigated to assess mechanical and structural properties of bone; however, bone matrix proteins and their discrepant regulation of diseased and aged bone are often overlooked. The current study considered the spine matrix properties of ovariectomized rats (OVX) against control rats (Sham) at 16 months of age. Diseased bone showed less compact structure with inhomogeneous distribution of type 1 collagen (Col1) and changes in osteocyte morphology. Intriguingly, demineralization patches were noticed in the vicinity of blood vessels in the OVX spine. The organic matrix structure was investigated using computational segmentation of collagen fibril properties. In contrast to the aged bone, diseased bone showed longer fibrils and smaller orientation angles. The study shows the potential of quantifying transmission electron microscopy images to predict the mechanical properties of bone tissue.
Databáze: MEDLINE
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