Intravital Microscopy of T-B Cell Interactions in Germinal Centers.
| Autor: | Shih C; Laboratory of Dynamic Immunobiology, Department of Basic Medical Sciences, Tsinghua-Peking Center for Life Sciences, Institute for Immunology, School of Medicine, Tsinghua University, Beijing, 100084, China., Qi H; Laboratory of Dynamic Immunobiology, Department of Basic Medical Sciences, Tsinghua-Peking Center for Life Sciences, Institute for Immunology, School of Medicine, Tsinghua University, Beijing, 100084, China. qihai@tsinghua.edu.cn. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2017; Vol. 1623, pp. 73-85. |
| DOI: | 10.1007/978-1-4939-7095-7_6 |
| Abstrakt: | Dynamic imaging analyses of antigen-specific T-B interactions in germinal centers have advanced our understanding of the molecular mechanisms underlying affinity maturation and provided a wealth of information about how follicular helper T cells function in vivo. Here we describe a routine method to visualize fluorescence protein-expressing, antigen-specific T and B cells in germinal centers. The protocol for incorporating functional reporters or genetic perturbation of the T cells by retroviral transduction is also briefly described, using the FRET-based calcium reporter as an example. |
| Databáze: | MEDLINE |
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