A new arginine-based dental adhesive system: formulation, mechanical and anti-caries properties.

Autor: Geraldeli S; Department of Restorative Dental Sciences, Division of Operative Dentistry, College of Dentistry, University of Florida, Gainesville, FL, USA., Soares EF; Department of Restorative Dentistry, Dental Materials Division, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil., Alvarez AJ; College of Dentistry, University of Florida, Gainesville, FL, USA., Farivar T; Department of Restorative Dental Sciences, Division of Operative Dentistry, College of Dentistry, University of Florida, Gainesville, FL, USA., Shields RC; Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL, USA., Sinhoreti MAC; Department of Restorative Dentistry, Dental Materials Division, Piracicaba Dental School, University of Campinas, Piracicaba, SP, Brazil., Nascimento MM; Department of Restorative Dental Sciences, Division of Operative Dentistry, College of Dentistry, University of Florida, Gainesville, FL, USA. Electronic address: mnascimento@dental.ufl.edu.
Jazyk: angličtina
Zdroj: Journal of dentistry [J Dent] 2017 Aug; Vol. 63, pp. 72-80. Date of Electronic Publication: 2017 Jun 03.
DOI: 10.1016/j.jdent.2017.05.024
Abstrakt: Secondary caries at the margins of composite restorations has been attributed to adhesive failure and consequent accumulation of cariogenic biofilms.
Objectives: To develop and evaluate an etch-and-rinse adhesive system containing arginine for sustainable release and recharge without affecting its mechanical properties. Arginine metabolism by oral bacteria generates ammonia, which neutralizes glycolytic acids and creates a neutral environmental pH that is less favorable to the growth of caries pathogens, thus reducing the caries risk at the tooth-composite interface.
Methods: Experimental adhesives were formulated with methacrylate monomers and arginine at 5%, 7%, and 10% or no arginine (control). Adhesives were tested for: (i) mechanical properties of true stress (FS and UTS), modulus of elasticity (E), degree of conversion (DC), Knoop hardness number (KHN) and dentin microtensile bond strength (μ-TBS), (ii) arginine release and recharge, and (iii) antibacterial activities. Data was analyzed by t-test, one-way ANOVA and Tukey's tests.
Results: FS and UTS results showed no statistically significant differences between the 7% arginine-adhesive and control, while the results for E, DC, KHN and μ-TBS showed no difference among all groups. The 7% arginine-adhesive showed a high release rate of arginine (75.0μmol/cm 2 ) at 2h, and a more sustainable, controlled release rate (up to 0.2μmol/cm 2 ) at 30days.
Conclusions: Incorporation of 7% arginine did not affect the physical and mechanical properties of the adhesive. Arginine was released from the adhesive at a rate and concentration that exhibited antibacterial effects, regardless of shifts in biofilm conditions such as sugar availability and pH.
Clinical Significance: Secondary caries is recognized as the main reason for failure of dental restorations. The development of an arginine-based adhesive system has the potential to dramatically reduce the incidence and severity of secondary caries in adhesive restorations in a very economical fashion.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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