SH3 Domain-Containing Protein 2 Plays a Crucial Role at the Step of Membrane Tubulation during Cell Plate Formation.

Autor:	Ahn G; Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea., Kim H; Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea., Kim DH; Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea., Hanh H; Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Korea., Yoon Y; Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois 60607., Singaram I; Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois 60607., Wijesinghe KJ; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556., Johnson KA; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556., Zhuang X; School of Life Sciences, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong 999077, China., Liang Z; School of Life Sciences, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong 999077, China., Stahelin RV; Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556.; Department of Biochemistry and Molecular Biology, Indiana University School of Medicine-South Bend, South Bend, Indiana 46617., Jiang L; School of Life Sciences, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong 999077, China., Cho W; Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois 60607., Kang BH; School of Life Sciences, Chinese University of Hong Kong, Shatin, New Territories, Hong Kong 999077, China., Hwang I; Department of Life Sciences, Pohang University of Science and Technology, Pohang 790-784, Korea ihhwang@postech.ac.kr.; Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, Pohang 790-784, Korea.
Jazyk:	angličtina
Zdroj:	The Plant cell [Plant Cell] 2017 Jun; Vol. 29 (6), pp. 1388-1405. Date of Electronic Publication: 2017 Jun 05.
DOI:	10.1105/tpc.17.00108
Abstrakt:	During cytokinesis in plants, trans -Golgi network-derived vesicles accumulate at the center of dividing cells and undergo various structural changes to give rise to the planar cell plate. However, how this conversion occurs at the molecular level remains elusive. In this study, we report that SH3 Domain-Containing Protein 2 (SH3P2) in Arabidopsis thaliana plays a crucial role in converting vesicles to the planar cell plate. SH3P2 RNAi plants showed cytokinesis-defective phenotypes and produced aggregations of vesicles at the leading edge of the cell plate. SH3P2 localized to the leading edge of the cell plate, particularly the constricted or curved regions of the cell plate. The BAR domain of SH3P2 induced tubulation of vesicles. SH3P2 formed a complex with dynamin-related protein 1A (DRP1A) and affected DRP1A accumulation to the cell plate. Based on these results, we propose that SH3P2 functions together with DRP1A to convert the fused vesicles to tubular structures during cytokinesis. (© 2017 American Society of Plant Biologists. All rights reserved.)
Databáze:	MEDLINE
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