Extracellular polysaccharide derived from potential probiotic strain with antioxidant and antibacterial activities as a prebiotic agent to control pathogenic bacterial biofilm formation.

Autor: Mahdhi A; Laboratoire d'analyse, de traitement et de valorisation des polluants de l'environnement et des produits, Faculté de Pharmacie de Monastir, Université de Monastir, Rue Ibn Sina, 5000 Monastir, Tunisia. Electronic address: abdelkarim_mh@yahoo.fr., Leban N; Laboratoire de biochimie et de Biologie moléculaire, Faculté de Pharmacie de Monastir, Université de Monastir, Rue Ibn Sina, 5000 Monastir, Tunisia., Chakroun I; Laboratoire d'analyse, de traitement et de valorisation des polluants de l'environnement et des produits, Faculté de Pharmacie de Monastir, Université de Monastir, Rue Ibn Sina, 5000 Monastir, Tunisia., Chaouch MA; Laboratoire des Interfaces et des Matériaux Avancés (LIMA), Faculté des Sciences de Monastir, Université de Monastir, Bd. de l'environnement, 5019 Monastir, Tunisia., Hafsa J; Laboratoire de Biochimie, Faculté de Médecine, Université de Sousse, 4002 Sousse, Tunisia., Fdhila K; Laboratoire d'analyse, de traitement et de valorisation des polluants de l'environnement et des produits, Faculté de Pharmacie de Monastir, Université de Monastir, Rue Ibn Sina, 5000 Monastir, Tunisia., Mahdouani K; Laboratoire d'analyse, de traitement et de valorisation des polluants de l'environnement et des produits, Faculté de Pharmacie de Monastir, Université de Monastir, Rue Ibn Sina, 5000 Monastir, Tunisia., Majdoub H; Laboratoire des Interfaces et des Matériaux Avancés (LIMA), Faculté des Sciences de Monastir, Université de Monastir, Bd. de l'environnement, 5019 Monastir, Tunisia. Electronic address: hatemmajdoub.fsm@gmail.com.
Jazyk: angličtina
Zdroj: Microbial pathogenesis [Microb Pathog] 2017 Aug; Vol. 109, pp. 214-220. Date of Electronic Publication: 2017 Jun 02.
DOI: 10.1016/j.micpath.2017.05.046
Abstrakt: Because of their functional diversity, bioactive compounds are becoming a new biocontrol agent to limit biofilm formation by pathogens. In this study, the physico-chemical characterization of an exopolysaccharide (EPS) isolated from Lactobacillus plantarum (EPLB) was characterized and its in vitro effect on biofilm formation was studied. The EPS had a molecular weight of 36 kDa and polydispersity index estimated to be 1.2. The tested EPLB had an antibacterial activity, with a Minimal Inhibition Concentration (MIC) values ranging between 1 mg/ml and 10 mg/ml, displayed an antibiofilm effect concentration dependent on Gram positive and negative strains. Among the pathogenic strains, 2 out of 4 appeared to be more than 50% inhibited in their biofilm development by the EPS. The antibiofilm activity can be due to the ability of the EPS to influence the function of biological membranes like hydrophobicity that decreased (P < 0.05) when the EPS was used at a concentration of 512 μg/ml. This EPS without cytotoxic effect, showed an antioxidant effect on the quenching of DPPH radicals and the inhibition of lipid peroxidation with a percentage of 64% and 66%, respectively. Taken together these biological properties, EPLB can be considered as a potential prebiotic agent in the design of new therapeutic strategies for bacterial biofilm-associated infections.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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