Examining the intersection between splicing, nuclear export and small RNA pathways.

Autor: Nabih A; Dept. of Molecular Genetics, University of Toronto, Canada., Sobotka JA; Dept. of Molecular Genetics, University of Toronto, Canada., Wu MZ; Dept. of Molecular Genetics, University of Toronto, Canada., Wedeles CJ; Dept. of Molecular Genetics, University of Toronto, Canada. Electronic address: chris.wedeles@mail.utoronto.ca., Claycomb JM; Dept. of Molecular Genetics, University of Toronto, Canada. Electronic address: julie.claycomb@utoronto.ca.
Jazyk: angličtina
Zdroj: Biochimica et biophysica acta. General subjects [Biochim Biophys Acta Gen Subj] 2017 Nov; Vol. 1861 (11 Pt B), pp. 2948-2955. Date of Electronic Publication: 2017 May 31.
DOI: 10.1016/j.bbagen.2017.05.027
Abstrakt: Background: Nuclear Argonaute/small RNA pathways in a variety of eukaryotic species are generally known to regulate gene expression via chromatin modulation and transcription attenuation in a process known as transcriptional gene silencing (TGS). However, recent data, including genetic screens, phylogenetic profiling, and molecular mechanistic studies, also point to a novel and emerging intersection between the splicing and nuclear export machinery with nuclear Argonaute/small RNA pathways in many organisms.
Scope of Review: In this review, we summarize the field's current understanding regarding the relationship between splicing, export and small RNA pathways, and consider the biological implications for coordinated regulation of transcripts by these pathways. We also address the importance and available approaches for understanding the RNA regulatory logic generated by the intersection of these particular pathways in the context of synthetic biology.
Major Conclusions: The interactions between various eukaryotic RNA regulatory pathways, particularly splicing, nuclear export and small RNA pathways provide a type of combinatorial code that informs the identity ("self" versus "non-self") and dictates the fate of each transcript in a cell. Although the molecular mechanisms for how splicing and nuclear export impact small RNA pathways are not entirely clear at this early stage, the links between these pathways are widespread across eukaryotic phyla.
General Significance: The link between splicing, nuclear export, and small RNA pathways is emerging and establishes a new frontier for understanding the combinatorial logic of gene regulation across species that could someday be harnessed for therapeutic, biotechnology and agricultural applications. This article is part of a Special Issue entitled "Biochemistry of Synthetic Biology - Recent Developments" Guest Editor: Dr. Ilka Heinemann and Dr. Patrick O'Donoghue.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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