Microbial metabolites profile during in vitro human colonic fermentation of breakfast menus consumed by Mexican school children.

Autor: Zamora-Gasga VM; Instituto Tecnológico de Tepic, Laboratorio Integral de Investigación en Alimentos, División de Estudios de Posgrado, Av Instituto Tecnológico No 2595, Col Lagos del Country, CP 63175 Tepic, Nayarit, Mexico., Montalvo-González E; Instituto Tecnológico de Tepic, Laboratorio Integral de Investigación en Alimentos, División de Estudios de Posgrado, Av Instituto Tecnológico No 2595, Col Lagos del Country, CP 63175 Tepic, Nayarit, Mexico., Loarca-Piña G; Programa de Posgrado en Alimentos del Centro de la República, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Cerro de las Campanas S/N, CP 76010, Santiago de Querétaro, Querétaro, Mexico., Vázquez-Landaverde PA; Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada del Instituto Politécnico Nacional, Unidad Querétaro, Cerro Blanco No. 141, Col Colinas del Cimatario, CP 76090, Santiago de Querétaro, Querétaro, Mexico., Tovar J; Food for Health Science Centre, Lund University, Medicon Village, Scheelevägen 2, Lund SE-223 81, Sweden., Sáyago-Ayerdi SG; Instituto Tecnológico de Tepic, Laboratorio Integral de Investigación en Alimentos, División de Estudios de Posgrado, Av Instituto Tecnológico No 2595, Col Lagos del Country, CP 63175 Tepic, Nayarit, Mexico. Electronic address: ssayago@ittepic.edu.mx.
Jazyk: angličtina
Zdroj: Food research international (Ottawa, Ont.) [Food Res Int] 2017 Jul; Vol. 97, pp. 7-14. Date of Electronic Publication: 2017 Mar 22.
DOI: 10.1016/j.foodres.2017.03.038
Abstrakt: The nutrition transition promotes the development of childhood obesity. Currently, Mexico is affected by this serious public health problem. The nutritional and functional characterization of a whole menu has a number of advantages over the study of single nutrients. Since breakfast is considered the most important meal of the day, this study aimed to evaluate the metabolite profile produced by in vitro human colonic fermentation of the isolated indigestible fraction (IF) from three different Mexican breakfast (M-B) menus (Modified "MM-B", traditional "TM-B", and alternative "AM-B"), previously identified as commonly consumed by Mexican schoolchildren in Nayarit State, Mexico. The M-B's consist of egg, corn tortilla, beans (higher in TM-B), sugar and chocolate powder (higher in AM-B) and milk, combined in different proportions. The IF in all breakfasts was about 4.7-5.6g/100g FW, with a relatively high content of protein (≈21%), which might have negative physiological implications. Fermentation of IF from TM-B resulted in the largest pH decrease after 72h (pH=6.07), with a low short chain fatty acid (SCFA) production (0.75 to 47.23mmol/L), but greater relative concentration of other fatty acids (FA) (C7, C8, C9). Besides, 55 volatile compounds were detected in the fermentation media by SPME-GC-MS and three principal components (PC) were identified. PC1 was influenced by SCFA production, low FA esters production (<8C), and low volatile organic acids production. PC2 was influenced by the decrease in pH and an increase in antioxidant capacity (p<0.0001). These results suggest that the production of different metabolites in the luminal medium may affect the pH and antioxidant status in the colon. Fermentation of IF from TM-M, assessed after 48 and 72h, showed the highest correlation for PC2; the metabolic pattern registered for this IF maybe considered beneficial.
(Copyright © 2017 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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