Human embryonic stem cell dispersion in electrospun PCL fiber scaffolds by coating with laminin-521 and E-cadherin-Fc.

Autor: Leino M; School of Biotechnology, Cell Technology Group (CETEG), KTH - Royal Institute of Technology, Stockholm, Sweden., Astrand C; School of Biotechnology, Cell Technology Group (CETEG), KTH - Royal Institute of Technology, Stockholm, Sweden., Hughes-Brittain N; The Electrospinning Company Ltd, R70 Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0QX, UK., Robb B; The Electrospinning Company Ltd, R70 Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0QX, UK., McKean R; The Electrospinning Company Ltd, R70 Rutherford Appleton Laboratory, Harwell Oxford, Didcot, Oxfordshire, OX11 0QX, UK., Chotteau V; School of Biotechnology, Cell Technology Group (CETEG), KTH - Royal Institute of Technology, Stockholm, Sweden.; AdBIOPRO, Competence Centre for Advanced Bioproduction by Continuous Bioprocessing, KTH, Stockholm, Sweden.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part B, Applied biomaterials [J Biomed Mater Res B Appl Biomater] 2018 Apr; Vol. 106 (3), pp. 1226-1236. Date of Electronic Publication: 2017 Jun 03.
DOI: 10.1002/jbm.b.33928
Abstrakt: Advances in human pluripotent cell cultivation and differentiation protocols have led to production of stem cell-derived progenitors as a promising cell source for replacement therapy. Three-dimensional (3-D) culture is a better mimic of the natural niche for stem cells and is widely used for disease modeling. Here, we describe a nonaggregate culture system of human embryonic stem cells inside electrospun polycaprolactone (PCL) fiber scaffolds combined with defined extracellular proteins naturally occurring in the stem cell niche. PCL fiber scaffolds coated with recombinant human laminin-521 readily supported initial stem cell attachment and growth from a single-cell suspension. The combination of recombinant E-cadherin-Fc and laminin-521 further improved cell dispersion rendering a uniform cell population. Finally, we showed that the cells cultured in E-cadherin-Fc- and laminin-521-coated PCL scaffolds could differentiate into all three germ layers. Importantly, we provided a chemically defined 3-D system in which pluripotent stem cells grown and differentiated avoiding the formation of cell aggregates. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1226-1236, 2018.
(© 2017 Wiley Periodicals, Inc.)
Databáze: MEDLINE
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