Anticonvulsive activity of (1S)-(-)-verbenone involving RNA expression of BDNF, COX-2, and c-fos.

Autor: de Melo CGF; Institute of Research in Drugs and Medicines, Federal University of Paraíba, CP 5009, João Pessoa, PB, 58051-900, Brazil., Salgado PRR; Institute of Research in Drugs and Medicines, Federal University of Paraíba, CP 5009, João Pessoa, PB, 58051-900, Brazil., da Fonsêca DV; Institute of Research in Drugs and Medicines, Federal University of Paraíba, CP 5009, João Pessoa, PB, 58051-900, Brazil., Braga RM; Institute of Research in Drugs and Medicines, Federal University of Paraíba, CP 5009, João Pessoa, PB, 58051-900, Brazil., Filho MRDC; Institute of Research in Drugs and Medicines, Federal University of Paraíba, CP 5009, João Pessoa, PB, 58051-900, Brazil., de Farias IEV; Institute of Research in Drugs and Medicines, Federal University of Paraíba, CP 5009, João Pessoa, PB, 58051-900, Brazil., de Luna Freire Pessôa H; Department of Cell and Molecular Biology, Federal University of Paraíba, CP 5009, João Pessoa, PB, 58051-900, Brazil., Lima EM; Department of Cell and Molecular Biology, Federal University of Paraíba, CP 5009, João Pessoa, PB, 58051-900, Brazil., do Amaral IPG; Department of Cell Biology and Molecular, Biotechnology Center (CBIOTEC) Federal University of Paraíba, CP 5009, João Pessoa, PB, 58051-900, Brazil., de Sousa DP; Institute of Research in Drugs and Medicines, Federal University of Paraíba, CP 5009, João Pessoa, PB, 58051-900, Brazil., de Almeida RN; Institute of Research in Drugs and Medicines, Federal University of Paraíba, CP 5009, João Pessoa, PB, 58051-900, Brazil. reinaldoan@uol.com.br.
Jazyk: angličtina
Zdroj: Naunyn-Schmiedeberg's archives of pharmacology [Naunyn Schmiedebergs Arch Pharmacol] 2017 Sep; Vol. 390 (9), pp. 863-869. Date of Electronic Publication: 2017 Jun 02.
DOI: 10.1007/s00210-017-1388-x
Abstrakt: (1S)-(-)-verbenone (VRB) is a monoterpene present in the essential oils of many plants which has shown therapeutic effect; however, its anticonvulsant activity has not yet been evaluated. The present work sought to investigate the anticonvulsant activity of VRB using pilocarpine and pentylenetetrazole-induced seizure testing; seeking also probable mechanisms of action. VRB caused no significant changes in motor coordination. Also, no significant data was observed in the pilocarpine-induced seizure tests. In the PTZ-induced seizures test, VRB showed anticonvulsant activity at doses of 200 mg/kg i.p. (733 ± 109.4 s) and 250 mg/kg i.p. (648.8 ± 124.5 s) significantly increasing the latency to onset of first seizure as compared with the vehicle group (51.8 ± 2.84 s). Pretreatment with flumazenil (FLU) did not reverse the anticonvulsive effect of VRB; however, it was able to upregulate BDNF and COX-2 genes and downregulate c-fos. The findings suggest that the anticonvulsant effects of VRB may be related to RNA expression modulations of COX-2, BDNF, and c-fos.
Databáze: MEDLINE
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