| Autor: |
Taitt CR; Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC United States of America., Leski TA; Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC United States of America., Erwin DP; US Army Medical Research Directorate-Kenya, Walter Reed Army Institute of Research, Kericho, Kenya., Odundo EA; KEMRI/US Army Medical Research Directorate-Kenya, Walter Reed Army Institute of Research, Kericho, Kenya., Kipkemoi NC; KEMRI/US Army Medical Research Directorate-Kenya, Walter Reed Army Institute of Research, Kericho, Kenya., Ndonye JN; KEMRI/US Army Medical Research Directorate-Kenya, Walter Reed Army Institute of Research, Kericho, Kenya., Kirera RK; KEMRI/US Army Medical Research Directorate-Kenya, Walter Reed Army Institute of Research, Kericho, Kenya., Ombogo AN; KEMRI/US Army Medical Research Directorate-Kenya, Walter Reed Army Institute of Research, Kericho, Kenya., Walson JL; Department of Global Health, University of Washington, Seattle, WA, United States of America.; Departments of Pediatrics, Medicine, and Epidemiology, University of Washington, Seattle, WA, United States of America., Pavlinac PB; Department of Global Health, University of Washington, Seattle, WA, United States of America., Hulseberg C; US Army Medical Research Directorate-Kenya, Walter Reed Army Institute of Research, Kericho, Kenya., Vora GJ; Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC United States of America. |
| Jazyk: |
angličtina |
| Zdroj: |
PloS one [PLoS One] 2017 Jun 02; Vol. 12 (6), pp. e0178880. Date of Electronic Publication: 2017 Jun 02 (Print Publication: 2017). |
| DOI: |
10.1371/journal.pone.0178880 |
| Abstrakt: |
We sought to determine the genetic and phenotypic antimicrobial resistance (AMR) profiles of commensal Klebsiella spp. circulating in Kenya by testing human stool isolates of 87 K. pneumoniae and three K. oxytoca collected at eight locations. Over one-third of the isolates were resistant to ≥3 categories of antimicrobials and were considered multidrug-resistant (MDR). We then compared the resistance phenotype to the presence/absence of 238 AMR genes determined by a broad-spectrum microarray and PCR. Forty-six genes/gene families were identified conferring resistance to β-lactams (ampC/blaDHA, blaCMY/LAT, blaLEN-1, blaOKP-A/OKP-B1, blaOXA-1-like family, blaOXY-1, blaSHV, blaTEM, blaCTX-M-1 and blaCTX-M-2 families), aminoglycosides (aac(3)-III, aac(6)-Ib, aad(A1/A2), aad(A4), aph(AI), aph3/str(A), aph6/str(B), and rmtB), macrolides (mac(A), mac(B), mph(A)/mph(K)), tetracyclines (tet(A), tet(B), tet(D), tet(G)), ansamycins (arr), phenicols (catA1/cat4, floR, cmlA, cmr), fluoroquinolones (qnrS), quaternary amines (qacEΔ1), streptothricin (sat2), sulfonamides (sul1, sul2, sul3), and diaminopyrimidines (dfrA1, dfrA5, dfrA7, dfrA8, dfrA12, dfrA13/21/22/23 family, dfrA14, dfrA15, dfrA16, dfrA17). This is the first profile of genes conferring resistance to multiple categories of antimicrobial agents in western and central Kenya. The large number and wide variety of resistance genes detected suggest the presence of significant selective pressure. The presence of five or more resistance determinants in almost two-thirds of the isolates points to the need for more effective, targeted public health policies and infection control/prevention measures. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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