| Autor: |
Siniscalchi LAB; Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais State, 31270-901, Brazil.; Department of Engineering, Federal University of Lavras, Dr. Sylvio Menicucci Avenue, 1001, Lavras, Minas Gerais State, 37200-000, Brazil., Leite LR; Department of Engineering, Federal University of Lavras, Dr. Sylvio Menicucci Avenue, 1001, Lavras, Minas Gerais State, 37200-000, Brazil., Oliveira G; Genomics and Computational Biology Group, René Rachou Research Center, Oswaldo Cruz Foundation, Augusto de Lima Avenue 1715, Belo Horizonte, Minas Gerais State, 30.190-002, Brazil.; Instituto Vale de Tecnologia, Rua Boaventura da Silva, 955, Belém, Pará, 66055-090, Brazil., Chernicharo CAL; Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais State, 31270-901, Brazil., de Araújo JC; Department of Sanitary and Environmental Engineering, Federal University of Minas Gerais, Antonio Carlos Avenue, 6627, Belo Horizonte, Minas Gerais State, 31270-901, Brazil. juliana@desa.ufmg.br. |
| Abstrakt: |
Methane is produced in anaerobic environments, such as reactors used to treat wastewaters, and can be consumed by methanotrophs. The composition and structure of a microbial community enriched from anaerobic sewage sludge under methane-oxidation condition coupled to denitrification were investigated. Denaturing gradient gel electrophoresis (DGGE) analysis retrieved sequences of Methylocaldum and Chloroflexi. Deep sequencing analysis revealed a complex community that changed over time and was affected by methane concentration. Methylocaldum (8.2%), Methylosinus (2.3%), Methylomonas (0.02%), Methylacidiphilales (0.45%), Nitrospirales (0.18%), and Methanosarcinales (0.3%) were detected. Despite denitrifying conditions provided, Nitrospirales and Methanosarcinales, known to perform anaerobic methane oxidation coupled to denitrification (DAMO) process, were in very low abundance. Results demonstrated that aerobic and anaerobic methanotrophs coexisted in the reactor together with heterotrophic microorganisms, suggesting that a diverse microbial community was important to sustain methanotrophic activity. The methanogenic sludge was a good inoculum to enrich methanotrophs, and cultivation conditions play a selective role in determining community composition. |
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