From Monochrome to Technicolor: Simple Generic Approaches to Multicomponent Protein Nanopatterning Using Siloxanes with Photoremovable Protein-Resistant Protecting Groups.

Autor:	El Zubir O; Department of Chemistry, University of Sheffield , Brook Hill, Sheffield S3 7HF, United Kingdom., Xia S; Department of Chemistry, University of Sheffield , Brook Hill, Sheffield S3 7HF, United Kingdom., Ducker RE; Department of Chemistry, University of Sheffield , Brook Hill, Sheffield S3 7HF, United Kingdom., Wang L; Department of Physics and Astronomy, University of Sheffield , Sheffield S3 7RH, United Kingdom., Mullin N; Department of Physics and Astronomy, University of Sheffield , Sheffield S3 7RH, United Kingdom., Cartron ML; Department of Molecular Biology and Biotechnology, University of Sheffield , Western Bank, Sheffield S10 2TN, United Kingdom., Cadby AJ; Department of Physics and Astronomy, University of Sheffield , Sheffield S3 7RH, United Kingdom., Hobbs JK; Department of Physics and Astronomy, University of Sheffield , Sheffield S3 7RH, United Kingdom., Hunter CN; Department of Molecular Biology and Biotechnology, University of Sheffield , Western Bank, Sheffield S10 2TN, United Kingdom., Leggett GJ; Department of Chemistry, University of Sheffield , Brook Hill, Sheffield S3 7HF, United Kingdom.
Jazyk:	angličtina
Zdroj:	Langmuir : the ACS journal of surfaces and colloids [Langmuir] 2017 Sep 05; Vol. 33 (35), pp. 8829-8837. Date of Electronic Publication: 2017 Jun 07.
DOI:	10.1021/acs.langmuir.7b01255
Abstrakt:	We show that sequential protein deposition is possible by photodeprotection of films formed from a tetraethylene-glycol functionalized nitrophenylethoxycarbonyl-protected aminopropyltriethoxysilane (NPEOC-APTES). Exposure to near-UV irradiation removes the protein-resistant protecting group, and allows protein adsorption onto the resulting aminated surface. The protein resistance was tested using proteins with fluorescent labels and microspectroscopy of two-component structures formed by micro- and nanopatterning and deposition of yellow and green fluorescent proteins (YFP/GFP). Nonspecific adsorption onto regions where the protecting group remained intact was negligible. Multiple component patterns were also formed by near-field methods. Because reading and writing can be decoupled in a near-field microscope, it is possible to carry out sequential patterning steps at a single location involving different proteins. Up to four different proteins were formed into geometric patterns using near-field lithography. Interferometric lithography facilitates the organization of proteins over square cm areas. Two-component patterns consisting of 150 nm streptavidin dots formed within an orthogonal grid of bars of GFP at a period of ca. 500 nm could just be resolved by fluorescence microscopy.
Databáze:	MEDLINE
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