The nuclear matrix protein CIZ1 facilitates localization of Xist RNA to the inactive X-chromosome territory.

Autor:	Ridings-Figueroa R; Department of Biology, University of York, York YO10 5DD, United Kingdom., Stewart ER; Department of Biology, University of York, York YO10 5DD, United Kingdom., Nesterova TB; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom., Coker H; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom., Pintacuda G; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom., Godwin J; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom., Wilson R; Department of Biology, University of York, York YO10 5DD, United Kingdom., Haslam A; Department of Biology, University of York, York YO10 5DD, United Kingdom., Lilley F; Department of Biology, University of York, York YO10 5DD, United Kingdom., Ruigrok R; Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM), University of Leeds, Leeds LS2 9JT, United Kingdom., Bageghni SA; Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM), University of Leeds, Leeds LS2 9JT, United Kingdom., Albadrani G; Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM), University of Leeds, Leeds LS2 9JT, United Kingdom.; Princess Nourah Bint Abdulrahman University (PNU), Riyadh, Kingdom of Saudi Arabia., Mansfield W; Stem Cell Institute, University of Cambridge, Cambridge CB2 1QR, United Kingdom., Roulson JA; Leeds Institute of Molecular Medicine (LIMM), University of Leeds, Leeds LS9 7TF, United Kingdom., Brockdorff N; Department of Biochemistry, University of Oxford, Oxford OX1 3QU, United Kingdom., Ainscough JFX; Department of Biology, University of York, York YO10 5DD, United Kingdom.; Leeds Institute of Cardiovascular and Metabolic Medicine (LICAMM), University of Leeds, Leeds LS2 9JT, United Kingdom., Coverley D; Department of Biology, University of York, York YO10 5DD, United Kingdom.
Jazyk:	angličtina
Zdroj:	Genes & development [Genes Dev] 2017 May 01; Vol. 31 (9), pp. 876-888. Date of Electronic Publication: 2017 May 25.
DOI:	10.1101/gad.295907.117
Abstrakt:	The nuclear matrix protein Cip1-interacting zinc finger protein 1 (CIZ1) promotes DNA replication in association with cyclins and has been linked to adult and pediatric cancers. Here we show that CIZ1 is highly enriched on the inactive X chromosome (Xi) in mouse and human female cells and is retained by interaction with the RNA-dependent nuclear matrix. CIZ1 is recruited to Xi in response to expression of X inactive-specific transcript (Xist) RNA during the earliest stages of X inactivation in embryonic stem cells and is dependent on the C-terminal nuclear matrix anchor domain of CIZ1 and the E repeats of Xist CIZ1-null mice, although viable, display fully penetrant female-specific lymphoproliferative disorder. Interestingly, in mouse embryonic fibroblast cells derived from CIZ1-null embryos, Xist RNA localization is disrupted, being highly dispersed through the nucleoplasm rather than focal. Focal localization is reinstated following re-expression of CIZ1. Focal localization of Xist RNA is also disrupted in activated B and T cells isolated from CIZ1-null animals, suggesting a possible explanation for female-specific lymphoproliferative disorder. Together, these findings suggest that CIZ1 has an essential role in anchoring Xist to the nuclear matrix in specific somatic lineages. (© 2017 Ridings-Figueroa et al.; Published by Cold Spring Harbor Laboratory Press.)
Databáze:	MEDLINE
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