Detection of intramyocardially injected DiR-labeled mesenchymal stem cells by optical and optoacoustic tomography.

Autor:	Berninger MT; Department of Orthopaedic Sports Medicine, Klinikum rechts der Isar, Technische Universität München, Munich, Germany.; Department of Trauma and Orthopaedic Surgery, BG Unfallklinik Murnau, Murnau, Germany., Mohajerani P; Institute for Biological and Medical Imaging, Technische Universität München und Helmholtz Zentrum München, Neuherberg, Germany., Wildgruber M; Department of Radiology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany., Beziere N; Institute for Biological and Medical Imaging, Technische Universität München und Helmholtz Zentrum München, Neuherberg, Germany., Kimm MA; Department of Radiology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany., Ma X; Institute for Biological and Medical Imaging, Technische Universität München und Helmholtz Zentrum München, Neuherberg, Germany., Haller B; Institute for Medical Statistics and Epidemiology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany., Fleming MJ; Department of Orthopaedic Sports Medicine, Klinikum rechts der Isar, Technische Universität München, Munich, Germany., Vogt S; Department of Orthopaedic Sports Medicine, Klinikum rechts der Isar, Technische Universität München, Munich, Germany., Anton M; Institute for Experimental Oncology and Therapy Research and Institute of Molecular Immunology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany., Imhoff AB; Department of Orthopaedic Sports Medicine, Klinikum rechts der Isar, Technische Universität München, Munich, Germany., Ntziachristos V; Institute for Biological and Medical Imaging, Technische Universität München und Helmholtz Zentrum München, Neuherberg, Germany., Meier R; Department of Radiology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany., Henning TD; Section of Neuroradiology, Uniklinik Köln, Cologne, Germany.
Jazyk:	angličtina
Zdroj:	Photoacoustics [Photoacoustics] 2017 May 04; Vol. 6, pp. 37-47. Date of Electronic Publication: 2017 May 04 (Print Publication: 2017).
DOI:	10.1016/j.pacs.2017.04.002
Abstrakt:	The distribution of intramyocardially injected rabbit MSCs, labeled with the near-infrared dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbo-cyanine-iodide (DiR) using hybrid Fluorescence Molecular Tomography-X-ray Computed Tomography (FMT-XCT) and Multispectral Optoacoustic Tomography (MSOT) imaging technologies, was investigated. Viability and induction of apoptosis of DiR labeled MSCs were assessed by XTT- and Caspase-3/-7-testing in vitro . 2 × 10 6 , 2 × 10 5 and 2 × 10 4 MSCs labeled with 5 and 10 μg DiR/ml were injected into fresh frozen rabbit hearts. FMT-XCT, MSOT and fluorescence cryosection imaging were performed. Concentrations up to 10 μg DiR/ml did not cause apoptosis in vitro (p > 0.05). FMT and MSOT imaging of labeled MSCs led to a strong signal. The imaging modalities highlighted a difference in cell distribution and concentration correlated to the number of injected cells. Ex-vivo cryosectioning confirmed the molecular fluorescence signal. FMT and MSOT are sensitive imaging techniques offering high-anatomic resolution in terms of detection and distribution of intramyocardially injected stem cells in a rabbit model.
Databáze:	MEDLINE
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