Neutralization of Inflammation by Inhibiting In vitro and In vivo Secretory Phospholipase A 2 by Ethanol Extract of Boerhaavia diffusa L.

Autor: Giresha AS; Department of Post Graduate Studies and Research in Biochemistry, Post Graduate Centre, Mangalore University, Kodagu, Karnataka, India., Pramod SN; Department of Studies and Research in Biochemistry, Laboratory of Immunomodulation and inflammation Biology, Sahyadri Science College (Autonomous), Kuvempu University, Shimoga, Karnataka, India., Sathisha AD; Department of Biochemistry, Institute of Biomedical Sciences, College of Health Sciences, Ayder Referral Hospital, Mekelle University, Mekelle, Ethiopia., Dharmappa KK; Department of Post Graduate Studies and Research in Biochemistry, Post Graduate Centre, Mangalore University, Kodagu, Karnataka, India.
Jazyk: angličtina
Zdroj: Pharmacognosy research [Pharmacognosy Res] 2017 Apr-Jun; Vol. 9 (2), pp. 174-181.
DOI: 10.4103/0974-8490.204650
Abstrakt: Background: Inflammation is a normal and necessary prerequisite to healing of the injured tissues. Inflammation contributes to all disease process including immunity, vascular pathology, trauma, sepsis, chemical, and metabolic injuries. The secretory phospholipase A 2 (sPLA 2 ) is a key enzyme in the production of pro-inflammatory mediators in chronic inflammatory disorders such as rheumatoid arthritis, coronary heart disease, diabetes, and asthma. The sPLA 2 also contribute to neuroinflammatory disorders such as Parkinson's, Alzheimer's, and Crohn's disease.
Aims: The present study aims to investigate the inhibition of human sPLA 2 by a popular medicinal herb Boerhaavia diffusa Linn. as a function of anti-inflammatory activity.
Materials and Methods: The aqueous and different organic solvents extracts of B. diffusa were prepared and evaluated for human synovial fluid, human pleural fluid, as well as Vipera russelli and Naja naja venom sPLA 2 enzyme inhibition.
Results: Among the extracts, the ethanol extract of B. diffusa (EEBD) showed the highest sPLA 2 inhibition and IC 50 values ranging from 17.8 to 27.5 μg. Further, antioxidant and lipid peroxidation activities of B. diffusa extract were checked using 2,2-diphenyl-1-picrylhydrazyl radical, thiobarbituric acid, and rat liver homogenate. The antioxidant activity of EEBD was more or less directly proportional to in vitro sPLA 2 inhibition. Eventually, the extract was subjected to neutralize sPLA 2 -induced mouse paw edema and indirect hemolytic activity. The EEBD showed similar potency in both the cases.
Conclusions: The findings suggest that the bioactive molecule/s from the EEBD is/are potentially responsible for the observed in vitro and in vivo sPLA 2 inhibition and antioxidant activity.
Summary: The present study aims to investigate the inhibition of human sPLA 2 by a popular medicinal herb Boerhaavia diffusa Linn. as a function of anti inflammatory activity. Abbreviation Used: EEBD: Ethanolic extract of boerhaavia diffusa, sPLA 2 : Secretory phospholipase A 2 , HSF: Human synovial fluid, HPF: Human pleural fluid, VRV-PLA2-V: Vipera russelli phospholipase A2, NN-PLA 2 -I: Naja naja phospholipase A 2 .
Competing Interests: There are no conflicts of interest.
Databáze: MEDLINE