| Autor: |
van Boheemen S; Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands., Tas A; Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands., Anvar SY; Department of Human Genetics, Leiden University Medical Center, Leiden, The Netherlands., van Grootveld R; Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands., Albulescu IC; Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands., Bauer MP; Department of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands., Feltkamp MC; Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands., Bredenbeek PJ; Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands., van Hemert MJ; Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands. M.J.van_Hemert@lumc.nl. |
| Abstrakt: |
The arthropod-borne Zika virus (ZIKV) is currently causing a major international public health threat in the Americas. This study describes the isolation of ZIKV from the plasma of a 29-year-old female traveler that developed typical symptoms, like rash, fever and headache upon return from Suriname. The complete genome sequence including the 5' and 3' untranslated regions was determined and phylogenetic analysis showed the isolate clustering within the Asian lineage, close to other viruses that have recently been isolated in the Americas. In addition, the viral quasispecies composition was analyzed by single molecule real time sequencing, which suggested a mutation frequency of 1.4 × 10 -4 for this ZIKV isolate. Continued passaging of the virus in cell culture led to the selection of variants with mutations in NS1 and the E protein. The latter might influence virus binding to cell surface heparan sulfate. |
