| Autor: |
Moore ES; Departments of Biomedical Sciences, Cornell Lab of Ornithology, Cornell University, Ithaca, New York;, Email: esm84@cornell.edu., Cleland TA; Departments of Psychology, Cornell Lab of Ornithology, Cornell University, Ithaca, New York., Williams WO; Center for Animal Resources and Education, Cornell Lab of Ornithology, Cornell University, Ithaca, New York., Peterson CM; Center for Animal Resources and Education, Cornell Lab of Ornithology, Cornell University, Ithaca, New York., Singh B; Center for Animal Resources and Education, Cornell Lab of Ornithology, Cornell University, Ithaca, New York., Southard TL; Departments of Biomedical Sciences, Cornell Lab of Ornithology, Cornell University, Ithaca, New York., Pasch B; Bioacoustics Research Program and Macaulay Library, Cornell Lab of Ornithology, Cornell University, Ithaca, New York, Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona., Labitt RN; College of Veterinary Medicine, Cornell University, Ithaca, New York., Daugherity EK; Departments of Biomedical Sciences, Center for Animal Resources and Education, Cornell Lab of Ornithology, Cornell University, Ithaca, New York. |
| Abstrakt: |
Tail tip amputation with minimal restraint is not widely used for mouse phlebotomy. In part, this infrequency may reflect policies influenced by tail tip amputation procedures for genotyping, which involve greater handling and tissue removal. To assess tail tip amputation with minimal restraint as a phlebotomy technique, we compared it with 2 more common methods: scruffing with facial vein puncture and lateral tail vein incision with minimal restraint. Blood glucose levels, audible and ultrasonic vocalizations, postphlebotomy activity and grooming behavior, open field and elevated plus maze behaviors, nest-building scores, and histologic changes at the phlebotomy site were evaluated. Mice in the facial vein phlebotomy group produced more audible vocalizations, exhibited lower postphlebotomy activity in the open field, and had more severe histologic changes than did mice in the tail incision and tail tip amputation groups. Facial vein phlebotomy did not affect grooming behavior relative to sham groups, whereas tail vein incision-but not tail tip amputation-increased tail grooming compared with that in control mice. Blood glucose levels, nest-building scores, and elevated plus maze behavior did not differ between groups, and no mice in any group produced ultrasonic vocalizations. Tail tip amputation mice did not perform differently than sham mice in any metric analyzed, indicating that this technique is a potentially superior method of blood collection in mice in terms of animal wellbeing. |
