Silencing of the Rice Gene LRR1 Compromises Rice Xa21 Transcript Accumulation and XA21-Mediated Immunity.

Autor: Caddell DF; Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, 95616, USA., Park CJ; Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, 95616, USA.; Present Address: Department of Bioresources Engineering and PERI, Sejong University, Seoul, 05006, Republic of Korea., Thomas NC; Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, 95616, USA.; Present Address: Department of Plant Pathology and Microbiology, University of California, Riverside, Riverside, CA, 92521, USA., Canlas PE; Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, 95616, USA., Ronald PC; Department of Plant Pathology and the Genome Center, University of California, Davis, Davis, CA, 95616, USA. pcronald@ucdavis.edu.
Jazyk: angličtina
Zdroj: Rice (New York, N.Y.) [Rice (N Y)] 2017 Dec; Vol. 10 (1), pp. 23. Date of Electronic Publication: 2017 May 22.
DOI: 10.1186/s12284-017-0162-5
Abstrakt: Background: The rice immune receptor XA21 confers resistance to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial leaf blight. We previously demonstrated that an auxilin-like protein, XA21 BINDING PROTEIN 21 (XB21), positively regulates resistance to Xoo.
Results: To further investigate the function of XB21, we performed a yeast two-hybrid screen. We identified 22 unique XB21 interacting proteins, including LEUCINE-RICH REPEAT PROTEIN 1 (LRR1), which we selected for further analysis. Silencing of LRR1 in the XA21 genetic background (XA21-LRR1Ri) compromises resistance to Xoo compared with control XA21 plants. XA21-LRR1Ri plants have reduced Xa21 transcript levels and reduced expression of genes that serve as markers of XA21-mediated activation. Overexpression of LRR1 is insufficient to alter resistance to Xoo in rice lines lacking XA21.
Conclusions: Taken together, our results indicate that LRR1 is required for wild-type Xa21 transcript expression and XA21-mediated immunity.
Databáze: MEDLINE
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