| Autor: |
Rubens CE; Department of Pediatrics, University of Washington, Seattle., Heggen LM |
| Jazyk: |
angličtina |
| Zdroj: |
Plasmid [Plasmid] 1988 Sep; Vol. 20 (2), pp. 137-42. |
| DOI: |
10.1016/0147-619x(88)90016-9 |
| Abstrakt: |
The tetracycline resistance gene encoded within the transposon Tn916 was replaced with the gene encoding erythromycin resistance from the plasmid pVA838. The derivative transposon of Tn916 was designated Tn916 delta E and was introduced into the Streptococcus faecalis chromosome by protoplast transformation. The conjugation/transposition functions of Tn916 delta E were similar to those observed for Tn916 in S. faecalis and Tn916 delta E was capable of self-conjugation at frequencies similar to those of other S. faecalis and Group B Streptococcus. This transposon will be useful for mutagenesis studies in gram-positive organisms, especially in those species where erythromycin resistance is a more desirable selectable marker. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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