Optimization of time for neural stem cells transplantation for brain stroke in rats.

Autor:	Ziaee SM; Student Research Committee, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran.; Stem Cell Laboratory, Department of Anatomy, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran.; Cell & Molecular Medicine Student Research Group, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran., Tabeshmehr P; Cell & Molecular Medicine Student Research Group, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran., Haider KH; Department of Basic Sciences, SRU, Saudi Arabia., Farrokhi M; Shiraz Neuroscience Research Center, Department of Neurology, Shiraz University of Medical Sciences, Shiraz, Iran.; Neurosurgery Department, Department of Neurology, Shiraz University of Medical Sciences, Shiraz, Iran., Shariat A; Shiraz Neuroscience Research Center, Department of Neurology, Shiraz University of Medical Sciences, Shiraz, Iran.; Clinical Neurology Research Center, Department of Neurology, Shiraz University of Medical Sciences, Shiraz, Iran., Amiri A; Shiraz Neuroscience Research Center, Department of Neurology, Shiraz University of Medical Sciences, Shiraz, Iran., Hosseini SM; Student Research Committee, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran.; Stem Cell Laboratory, Department of Anatomy, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran.; Cell & Molecular Medicine Student Research Group, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran.
Jazyk:	angličtina
Zdroj:	Stem cell investigation [Stem Cell Investig] 2017 Apr 14; Vol. 4, pp. 29. Date of Electronic Publication: 2017 Apr 14 (Print Publication: 2017).
DOI:	10.21037/sci.2017.03.10
Abstrakt:	Background: Despite encouraging data in terms of neurological outcome, stem cell based therapy for ischemic stroke in experimental models and human patients is still hampered by multiple as yet un-optimized variables, i.e., time of intervention, that significantly influence the prognosis. The aim of the present study was to delineate the optimum time for neural stem cells (NSCs) transplantation after ischemic stroke. Methods: The NSCs were isolated from 14 days embryo rat ganglion eminence and were cultured in NSA medium (neurobasal medium, 2% B27, 1% N2, bFGF 10 ng/mL, EGF 20 ng/mL and 1% pen/strep). The cells were characterized for tri-lineage differentiation by immunocytochemistry for tubulin-III, Olig2 and GFAP expression for neurons, oligodendrocytes and astrocyte respectively. The NSCs at passage 3 were injected intraventricularly in a rodent model of middle-cerebral artery occlusion (MCAO) on stipulated time points of 1 & 12 h, and 1, 3, 5 and 7 days after ischemic stroke. The animals were euthanized on day 28 after their respective treatment. Results: dUTP nick end labeling (TUNEL) assay and Caspase assay showed significantly reduced number of apoptotic cells on day 3 treated animals as compared to the other treatment groups of animals. The neurological outcome showed that the group which received NSCs 3 days after brain ischemia had the best neurological performance. Conclusions: The optimum time for NSCs transplantation was day 3 after ischemic stroke in terms of attenuation of ischemic zone expansion and better preserved neurological performance. Competing Interests: Conflicts of Interest: The author has no conflicts of interest to declare.
Databáze:	MEDLINE
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