Evaluation of direct and cell-mediated triple-gene therapy in spinal cord injury in rats.

Autor: Islamov RR; Kazan State Medical University, Kazan, Russia., Izmailov AA; Kazan State Medical University, Kazan, Russia., Sokolov ME; Kazan State Medical University, Kazan, Russia., Fadeev PO; Kazan State Medical University, Kazan, Russia., Bashirov FV; Kazan State Medical University, Kazan, Russia., Eremeev AA; Kazan Federal University, Kazan, Russia., Shaymardanova GF; Kazan State Medical University, Kazan, Russia., Shmarov MM; Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia., Naroditskiy BS; Gamaleya Research Institute of Epidemiology and Microbiology, Moscow, Russia., Chelyshev YA; Kazan State Medical University, Kazan, Russia., Lavrov IA; Kazan Federal University, Kazan, Russia; Department of Neurologic Surgery, Mayo Clinic, Rochester, MN, USA., Palotás A; Kazan Federal University, Kazan, Russia; Asklepios-Med (Private Medical Practice and Research Center), Szeged, Hungary. Electronic address: palotas@asklepios-med.eu.
Jazyk: angličtina
Zdroj: Brain research bulletin [Brain Res Bull] 2017 Jun; Vol. 132, pp. 44-52. Date of Electronic Publication: 2017 May 18.
DOI: 10.1016/j.brainresbull.2017.05.005
Abstrakt: Current treatment options for spinal cord injury (SCI) are scarce. One of the most promising innovative approaches include gene-therapy, however no single gene has so far been shown to be of clinical relevance. This study investigates the efficacy of various combinations of vascular endothelial growth factor (VEGF), glial cell-derived neurotrophic factor (GDNF), angiogenin (ANG) and neuronal cell adhesion molecule (NCAM) in rats. Multiple therapeutic genes were administered intrathecally either via adenoviral vectors or by using genetically modified human umbilical cord blood mononuclear cells (hUCBMCs). Following the induction of SCI, serial assessment of cord regeneration was performed, including morphometric analysis of gray and white matters, electrophysiology and behavioral test. The therapeutic gene combinations VEGF+GDNF+NCAM and VEGF+ANG+NCAM had positive outcomes on spinal cord regeneration, with enhanced recovery seen by the cell-based approach when compared to direct gene therapy. The efficacy of the genes and the delivery methods are discussed in this paper, recommending their potential use in SCI.
(Copyright © 2017 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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