| Autor: |
Todescato D; Laboratório de Transferência de Massa, Departamento de Engenharia Química e Engenharia de Alimentos, Universidade Federal de Santa Catarina, PO Box 476, Florianopolis, SC, 88040-900, Brazil., Maass D; Laboratório de Transferência de Massa, Departamento de Engenharia Química e Engenharia de Alimentos, Universidade Federal de Santa Catarina, PO Box 476, Florianopolis, SC, 88040-900, Brazil., Mayer DA; Laboratório de Transferência de Massa, Departamento de Engenharia Química e Engenharia de Alimentos, Universidade Federal de Santa Catarina, PO Box 476, Florianopolis, SC, 88040-900, Brazil., Vladimir Oliveira J; Laboratório de Transferência de Massa, Departamento de Engenharia Química e Engenharia de Alimentos, Universidade Federal de Santa Catarina, PO Box 476, Florianopolis, SC, 88040-900, Brazil., de Oliveira D; Laboratório de Transferência de Massa, Departamento de Engenharia Química e Engenharia de Alimentos, Universidade Federal de Santa Catarina, PO Box 476, Florianopolis, SC, 88040-900, Brazil. debora.oliveira@ufsc.br., Ulson de Souza SMAG; Laboratório de Transferência de Massa, Departamento de Engenharia Química e Engenharia de Alimentos, Universidade Federal de Santa Catarina, PO Box 476, Florianopolis, SC, 88040-900, Brazil., Ulson de Souza AA; Laboratório de Transferência de Massa, Departamento de Engenharia Química e Engenharia de Alimentos, Universidade Federal de Santa Catarina, PO Box 476, Florianopolis, SC, 88040-900, Brazil. |
| Abstrakt: |
Rhodococcus sp. has a broad catabolic diversity and unique enzymatic capabilities, and it is able to adapt under extreme conditions. Thereby, the production of this remarkable bacterium has a great biotechnological and industrial importance. In this sense, we sought to improve the R. erythropolis ATCC 4277 growth through a central composite design, by varying the components of nutrient medium (glucose, malt extract, yeast extract, CaCO 3 ), temperature, and agitation. It was found that the concentrations of glucose and malt extract are not statistically significant, being reduced of 4.0 and 10.0 g L -1 to 2.0 and 5.0 g L -1 , respectively. The CaCO 3 concentration and temperature were also diminished of 2.0 to 1.16 g L -1 and 28 to 23.7 °C, respectively. Optimal growth conditions provided a 240% increase in final biomass concentration, an increment in specific growth rate, and a growth yield coefficient about five times greater. Application of the optimal conditions in biodesulfurization and biodenitrogenation processes showed that desulfurization capability is not associated with optimal growth conditions; however, it was achieved a 47% of nitrogen removal in the assay containing 10% (w/w) of heavy gas oil. Graphical Abstract ᅟ. |
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