| Autor: |
Savolainen KE; Orion Corporation, Ltd., Orion Pharmaceutica, Kuopio, Finland., Pynnönen KM, Lapinjoki SP, Vidgren MT |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of pharmaceutical sciences [J Pharm Sci] 1988 Sep; Vol. 77 (9), pp. 802-3. |
| DOI: |
10.1002/jps.2600770918 |
| Abstrakt: |
A rapid and precise method for the determination of fat-soluble vitamins from a water-based multivitamin mixture was developed utilizing solid-phase extraction and reversed-phase high-performance liquid chromatography (HPLC). Cholecalciferol, alpha-tocopherol acetate, and retinol palmitate were extracted in a single stage from the matrix using Bond Elut C18 columns. The vitamins were then chromatographed on a Hypersil 5-microns C18 column, using a water:methanol gradient, and quantified simultaneously using individual UV absorption maxima. The recovery of added analytes varied from 92.6 to 100.6%. The assay coefficient of variation was 1.7-2.7%. The sample preparation method and HPLC assay described here are practical for pharmaceutical quality control purposes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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