| Autor: |
Jacobsen KL; Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602., Rockwood GA, Abolhassani M, Evans DL, Chitwood SW, Charamella L |
| Jazyk: |
angličtina |
| Zdroj: |
American journal of veterinary research [Am J Vet Res] 1988 Sep; Vol. 49 (9), pp. 1441-6. |
| Abstrakt: |
Kinetics of large-scale production of naturally derived bovine leukocyte interferon (IFN) was investigated using Sendai virus, Newcastle disease virus, and infectious bovine rhinotracheitis virus inducers. Cultures were tested for IFN production every 6 hours for 66 hours. The effect of varying the priming dose of Sendai virus from 0 to 50% of total virus dose and the effect of varying the priming time from 0 to 4 hours before induction also were investigated. Other factors explored were effects of varying the fetal bovine serum concentration (from 0 to 8%) and individual cow donors on bovine IFN titers. Highest bovine leukocyte IFN titers (15,314 U/ml) were obtained using Sendai virus (priming dose, 60 hemagglutinating units/ml; inducing dose, 240 hemagglutinating units/ml) and incubating for 12 hours. Up to 24 L (over 360 million U) of naturally derived leukocyte IFN were produced at one time. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
