Diversity, in-vitro virulence traits and antifungal susceptibility pattern of gastrointestinal yeast flora of healthy poultry, Gallus gallus domesticus.

Autor: Subramanya SH; Manipal College of Medical Sciences, Pokhara, Nepal. supram.gowda@gmail.com., Sharan NK; Manipal College of Medical Sciences, Pokhara, Nepal., Baral BP; Manipal College of Medical Sciences, Pokhara, Nepal., Hamal D; Manipal College of Medical Sciences, Pokhara, Nepal., Nayak N; Manipal College of Medical Sciences, Pokhara, Nepal., Prakash PY; Kasturba Medical College, Manipal University, Udupi, India., Sathian B; Manipal College of Medical Sciences, Pokhara, Nepal., Bairy I; Melaka-Manipal Medical College, Manipal University, Udupi, India., Gokhale S; Manipal College of Medical Sciences, Pokhara, Nepal.
Jazyk: angličtina
Zdroj: BMC microbiology [BMC Microbiol] 2017 May 15; Vol. 17 (1), pp. 113. Date of Electronic Publication: 2017 May 15.
DOI: 10.1186/s12866-017-1024-4
Abstrakt: Background: Poultry farming and consumption of poultry (Gallus gallus domesticus) meat and eggs are common gastronomical practices worldwide. Till now, a detailed understanding about the gut colonisation of Gallus gallus domesticus by yeasts and their virulence properties and drug resistance patterns in available literature remain sparse. This study was undertaken to explore this prevalent issue.
Results: A total of 103 specimens of fresh droppings of broiler chickens (commercial G domesticus) and domesticated chickens (domesticated G domesticus) were collected from the breeding sites. The isolates comprised of 29 (33%) Debaryozyma hansenii (Candida famata), 12 (13.6%) Sporothrix catenata (C. ciferrii), 10 (11.4%) C. albicans, 8 (9.1%) Diutnia catenulata (C. catenulate), 6 (6.8%) C. tropicalis, 3 (3.4%) Candida acidothermophilum (C. krusei), 2 (2.3%) C. pintolopesii, 1 (1.1%) C. parapsilosis, 9 (10.2%) Trichosporon spp. (T. moniliiforme, T. asahii), 4 (4.5%) Geotrichum candidum, 3 (3.4%) Cryptococcus macerans and 1 (1%) Cystobasidium minuta (Rhodotorula minuta). Virulence factors, measured among different yeast species, showed wide variability. Biofilm cells exhibited higher Minimum Inhibitory Concentration (MIC) values (μg/ml) than planktonic cells against all antifungal compounds tested: (fluconazole, 8-512 vs 0.031-16; amphotericin B, 0.5-64 vs 0.031-16; voriconazole 0.062-16 vs 0.062-8; caspofungin, 0.062-4 vs 0.031-1).
Conclusions: The present work extends the current understanding of in vitro virulence factors and antifungal susceptibility pattern of gastrointestinal yeast flora of G domesticus. More studies with advanced techniques are needed to quantify the risk of spread of these potential pathogens to environment and human.
Databáze: MEDLINE
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