Comparison of humoral and T-cell-mediated immune responses to a single dose of Bovela ® live double deleted BVDV vaccine or to a field BVDV strain.

Autor: Platt R; College of Veterinary Medicine, Iowa State University, Ames, IA, USA., Kesl L; Veterinary Resources, Inc., Ames, IA, USA., Guidarini C; Boehringer Ingelheim Animal Health GmbH, Ingelheim am Rhein, Germany., Wang C; College of Veterinary Medicine, Iowa State University, Ames, IA, USA., Roth JA; College of Veterinary Medicine, Iowa State University, Ames, IA, USA. Electronic address: jaroth@iastate.edu.
Jazyk: angličtina
Zdroj: Veterinary immunology and immunopathology [Vet Immunol Immunopathol] 2017 May; Vol. 187, pp. 20-27. Date of Electronic Publication: 2017 Mar 19.
DOI: 10.1016/j.vetimm.2017.03.003
Abstrakt: The objective of this study was to determine and compare the humoral and cellular immune responses of calves exposed to a single dose of Bovela ® bovine viral diarrhea virus (BVDV) live double deleted vaccine or a field strain virus (FSV) of BVDV type 2 (strain 890). Thirty seronegative, colostrum-deprived 5 month-old Holstein steer calves that tested negative for persistent BVDV by ear notch immunohistochemistry and seronegative to BVDV types 1 and 2 were used. Calves were screened by multi-parameter flow cytometry (MP-FCM) 1 week before vaccination to ensure that they were negative for T cell responses to the BVDV types 1 and 2 viruses in the Bovela ® vaccine. Calves were assigned to 3 treatment groups: control (PBS), FSV inoculated, and Bovela ® vaccinated. The humoral response was tested by standard serum virus neutralization (SVN) test to BVDV types 1 (Singer strain) and 2 (strain 125). The response by CD4, CD8, and gamma delta (γδ TCR) T cells was evaluated by MP-FCM using individual BVDV types 1 and 2 from Bovela ® vaccine as recall antigens at 5, 6, and 7 weeks after vaccination. Activation markers used were upregulation of surface CD25 (IL-2R), intracellular interferon gamma (IFNγ) and intracellular interleukin 4 (IL-4). Each T cell subset was evaluated for increased expression of each activation marker compared to non-antigen stimulated cells of the same animal. All Bovela ® vaccinated and FSV inoculated calves produced SVN antibodies to both BVDV types 1 and 2 while control animals remained seronegative throughout the study. The mean (weeks 5, 6, and 7) T cell recall responses to Bovela ® BVDV type 1 and type 2 recall antigens were numerically higher in all three T cell subsets (CD4, CD8, and γδ TCR) for all three activation markers (CD25, IFNγ, and IL-4) when compared to either the control animals or to the FSV inoculated animals. These differences were often, but not always, statistically significant (P<0.05).
(Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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