Quercetin Attenuates Benzo(α)pyrene-induced CYP1A Expression.

Autor: Perepechaeva ML; Institute of Molecular Biology and Biophysics, Novosibirsk 630117, Russia., Seredina TA; Institute of Molecular Biology and Biophysics, Novosibirsk 630117, Russia., Sidorova YA; Institute of Molecular Biology and Biophysics, Novosibirsk 630117, Russia., Pivovarova EN; Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk 630090, Russia., Markel AL; Federal research center Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences (SB RAS), Novosibirsk 630090, Russia; Novosibirsk State University, Novosibirsk 630090, Russia., Lyakhovich VV; Institute of Molecular Biology and Biophysics, Novosibirsk 630117, Russia; Novosibirsk State University, Novosibirsk 630090, Russia., Grishanova AY; Institute of Molecular Biology and Biophysics, Novosibirsk 630117, Russia.
Jazyk: angličtina
Zdroj: Biomedical and environmental sciences : BES [Biomed Environ Sci] 2017 Apr; Vol. 30 (4), pp. 308-313.
DOI: 10.3967/bes2017.041
Abstrakt: We studied effects of nutrient quercetin on cytochromes' Р450 1А (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities).
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Databáze: MEDLINE
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