Electrospun ultrathin PBAT/nHAp fibers influenced the in vitro and in vivo osteogenesis and improved the mechanical properties of neoformed bone.

Autor: Santana-Melo GF; Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Sao Paulo State University, Sao Jose dos Campos, Sao Paulo, Brazil., Rodrigues BVM; Laboratory of Biomedical Nanotechnology, Universidade Brasil, Itaquera, Sao Paulo, Brazil., da Silva E; Laboratory of Biomedical Nanotechnology, Institute of Research and Development (IP&D), University of Vale do Paraiba, Sao Jose dos Campos, Sao Paulo, Brazil., Ricci R; Laboratory of Biomedical Nanotechnology, Institute of Research and Development (IP&D), University of Vale do Paraiba, Sao Jose dos Campos, Sao Paulo, Brazil., Marciano FR; Laboratory of Biomedical Nanotechnology, Universidade Brasil, Itaquera, Sao Paulo, Brazil; Laboratory of Biomedical Nanotechnology, Institute of Research and Development (IP&D), University of Vale do Paraiba, Sao Jose dos Campos, Sao Paulo, Brazil; Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, USA; Nanomedicine Laboratory, Department of Chemical Engineering, Northeastern University, Boston, MA, USA., Webster TJ; Nanomedicine Laboratory, Department of Chemical Engineering, Northeastern University, Boston, MA, USA., Vasconcellos LMR; Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, Sao Paulo State University, Sao Jose dos Campos, Sao Paulo, Brazil. Electronic address: luana@ict.unesp.br., Lobo AO; Laboratory of Biomedical Nanotechnology, Universidade Brasil, Itaquera, Sao Paulo, Brazil; Laboratory of Biomedical Nanotechnology, Institute of Research and Development (IP&D), University of Vale do Paraiba, Sao Jose dos Campos, Sao Paulo, Brazil; Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, USA; Nanomedicine Laboratory, Department of Chemical Engineering, Northeastern University, Boston, MA, USA. Electronic address: aolobo@mit.edu.
Jazyk: angličtina
Zdroj: Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2017 Jul 01; Vol. 155, pp. 544-552. Date of Electronic Publication: 2017 Apr 27.
DOI: 10.1016/j.colsurfb.2017.04.053
Abstrakt: Combining polyester scaffolds with synthetic nanohydroxyapatite (nHAp), which is bioactive and osteoconductive, is a plausible strategy to improve bone regeneration. Here, we propose the combination of PBAT [poly(butylene-adipate-co-terephthalate)] and synthetic nHAp (at 3 and 5wt%). PBAT is a relatively a new polymer with low crystallinity and attractive biodegradability and mechanical properties for orthopedic applications, however, with a still underexplored potential for in vivo applications. Then, we performed a careful biological in vitro and in vivo set of experiments to evaluate the influence of PBAT containing two different nHAp loads. For in vitro assays, osteoblast-like MG63 cells were used and the bioactivity and gene expression related to osteogenesis were evaluated by qRT-PCR. For in vivo experiments, twenty-four male rats were used and a tibial defect model was applied to insert the scaffolds. Micro-computed tomography (Micro-CT) and histological analysis were used to assess e bone neoformation after 6 weeks of implantation. Three point flexural tests measured the mechanical properties of the neoformed bone. All scaffolds showed promising in vitro properties, since they were not cytotoxic against MG-63 cells and promoted high cell proliferation and formation of mineralized nodules. From a mechanistic point-of-view, nHAp loading increased hydrophilicity, which in turn allowed for a better adsorption of proteins and consequent changes in the phenotypic expression of osteoblasts. nHAp induced better cellular responses on/in the scaffolds, which was mainly attributed to its osteoconductive and osteoinductive properties. Micro-CT images showed that nHAp at 3% and 5wt% led to more effective bone formation, presenting the highest bone volume after 6 weeks of implantation. Considering the three point flexural tests, 5wt% of nHAp positively influenced the flexural mode of the neoformed bone, but the stiffiness was similar between the 3% and 5wt% groups. In summary, this investigation demonstrated great potential for the application of these novel scaffolds towards bone regeneration and, thus, should be further studied.
(Copyright © 2017 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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