The effect of biological sealants and adhesive treatments on matrix metalloproteinase expression during renal injury healing.

Autor: Lloris-Carsí JM; Department of Surgery, University of Valencia, Valencia, Spain., Barrios C; Division of Experimental Surgery, Valencia Catholic University, Valencia, Spain., Prieto-Moure B; Division of Experimental Surgery, Valencia Catholic University, Valencia, Spain., Lloris-Cejalvo JM; Division of Experimental Surgery, Valencia Catholic University, Valencia, Spain., Cejalvo-Lapeña D; Division of Experimental Surgery, Valencia Catholic University, Valencia, Spain.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2017 May 11; Vol. 12 (5), pp. e0177665. Date of Electronic Publication: 2017 May 11 (Print Publication: 2017).
DOI: 10.1371/journal.pone.0177665
Abstrakt: Background: Renal injuries are relatively common in cases of abdominal trauma. Adhesives and sealants can be used to repair and preserve damaged organs. Using a rat model, this study explores the activity of different matrix metalloproteinases (MMP) during the healing of renal injuries treated by two biological adhesives (TachoSil and GelitaSpon) and a new synthetic elastic cyanoacrylate (Adhflex).
Methods: Renal traumatic injuries were experimentally induced in 90 male Wistar rats by a Stiefel Biopsy Punch in the anterior aspect of the left kidney. Animals were divided into five groups: 1, sham non-injured (n = 3); 2, non-treated standard punch injury (n = 6); 3, punch injury treated with TachoSil (n = 27); 4, punch injury treated with GelitaSpon (n = 27); and, 5, punch injury treated with Adhflex (n = 27). Wound healing was evaluated 2, 6, and 18 days after injury by determining the expression of MMPs, and the histopathological evolution of lesions.
Findings: Histologically, the wound size at 6 days post-injury was larger in Adhflex-treated samples than in the other treatments, but the scarring tissue was similar at 18 days post-injury. Only the MMPs subtypes 1, 2, 8, 9, and 13 were sufficiently expressed to be quantifiable. Both time since injury and treatment type had a significant influence on MMPs expression. Two days after injury, the expression of MMP8 and MMP9 was predominant. MMP2 expression was greater 6 days after injury. The Adhflex-treated group had a significantly higher MMPs expression than the other treatment groups at all healing stages.
Conclusions: All three sealant treatments induced almost similar expression of MMPs than untreated animals indicating a physiological healing process. Given that all renal trauma injuries must be considered emergencies, both biological and synthetic adhesives, such as Adhflex, should be considered as a treatment options.
Databáze: MEDLINE
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