| Autor: |
Yoo YH; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee UniversityYongin, South Korea., Nalini Chandran AK; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee UniversityYongin, South Korea., Park JC; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee UniversityYongin, South Korea., Gho YS; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee UniversityYongin, South Korea., Lee SW; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee UniversityYongin, South Korea., An G; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee UniversityYongin, South Korea., Jung KH; Graduate School of Biotechnology & Crop Biotech Institute, Kyung Hee UniversityYongin, South Korea. |
| Jazyk: |
angličtina |
| Zdroj: |
Frontiers in plant science [Front Plant Sci] 2017 Apr 26; Vol. 8, pp. 580. Date of Electronic Publication: 2017 Apr 26 (Print Publication: 2017). |
| DOI: |
10.3389/fpls.2017.00580 |
| Abstrakt: |
Water deficiencies are one of the most serious challenges to crop productivity. To improve our understanding of soil moisture stress, we performed RNA-Seq analysis using roots from 4-week-old rice seedlings grown in soil that had been subjected to drought conditions for 2-3 d. In all, 1,098 genes were up-regulated in response to soil moisture stress for 3 d, which causes severe damage in root development after recovery, unlikely that of 2 d. Comparison with previous transcriptome data produced in drought condition indicated that more than 68% of our candidate genes were not previously identified, emphasizing the novelty of our transcriptome analysis for drought response in soil condition. We then validated the expression patterns of two candidate genes using a promoter-GUS reporter system in planta and monitored the stress response with novel molecular markers. An integrating omics tool, MapMan analysis, indicated that RING box E3 ligases in the ubiquitin-proteasome pathways are significantly stimulated by induced drought. We also analyzed the functions of 66 candidate genes that have been functionally investigated previously, suggesting the primary roles of our candidate genes in resistance or tolerance relating traits including drought tolerance (29 genes) through literature searches besides diverse regulatory roles of our candidate genes for morphological traits (15 genes) or physiological traits (22 genes). Of these, we used a T-DNA insertional mutant of rice phytochrome B (OsPhyB) that negatively regulates a plant's degree of tolerance to water deficiencies through the control of total leaf area and stomatal density based on previous finding. Unlike previous result, we found that OsPhyB represses the activity of ascorbate peroxidase and catalase mediating reactive oxygen species (ROS) processing machinery required for drought tolerance of roots in soil condition, suggesting the potential significance of remaining uncharacterized candidate genes for manipulating drought tolerance in rice. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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