Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein.

Autor:	Tabib-Salazar A; MRC Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, UK., Liu B; MRC Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, UK., Shadrin A; G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow 142290, Russia., Burchell L; MRC Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, UK., Wang Z; MRC Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, UK., Wang Z; MRC Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, UK., Goren MG; Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel., Yosef I; Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel., Qimron U; Department of Clinical Microbiology and Immunology, Sackler School of Medicine, Tel Aviv University, Tel Aviv 69978, Israel., Severinov K; Waksman Institute of Microbiology, Rutgers, The State University of New Jersey, 190 Frelinghuysen Road, Piscataway, NJ 08854-8020, USA., Matthews SJ; MRC Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, UK., Wigneshweraraj S; MRC Centre for Molecular Microbiology and Infection, Imperial College London, London SW7 2AZ, UK.
Jazyk:	angličtina
Zdroj:	Nucleic acids research [Nucleic Acids Res] 2017 Jul 27; Vol. 45 (13), pp. 7697-7707.
DOI:	10.1093/nar/gkx370
Abstrakt:	Infection of Escherichia coli by the T7 phage leads to rapid and selective inhibition of the bacterial RNA polymerase (RNAP) by the 7 kDa T7 protein Gp2. We describe the identification and functional and structural characterisation of a novel 7 kDa T7 protein, Gp5.7, which adopts a winged helix-turn-helix-like structure and specifically represses transcription initiation from host RNAP-dependent promoters on the phage genome via a mechanism that involves interaction with DNA and the bacterial RNAP. Whereas Gp2 is indispensable for T7 growth in E. coli, we show that Gp5.7 is required for optimal infection outcome. Our findings provide novel insights into how phages fine-tune the activity of the host transcription machinery to ensure both successful and efficient phage progeny development. (© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu