| Autor: |
Liu XW; 1 Institute of Anatomy, Histology, Embryology, and Neuroscience, School of Basic Medical Sciences, Lanzhou University , Lanzhou, P.R. China ., Yin J; 1 Institute of Anatomy, Histology, Embryology, and Neuroscience, School of Basic Medical Sciences, Lanzhou University , Lanzhou, P.R. China ., Ma QS; 2 Department of Basic Medicine, Medical College of Qinghai University , Xining, P.R. China ., Qi CC; 1 Institute of Anatomy, Histology, Embryology, and Neuroscience, School of Basic Medical Sciences, Lanzhou University , Lanzhou, P.R. China ., Mu JY; 1 Institute of Anatomy, Histology, Embryology, and Neuroscience, School of Basic Medical Sciences, Lanzhou University , Lanzhou, P.R. China ., Zhang L; 1 Institute of Anatomy, Histology, Embryology, and Neuroscience, School of Basic Medical Sciences, Lanzhou University , Lanzhou, P.R. China ., Gao LP; 3 Institute of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Lanzhou University , Lanzhou, P.R. China ., Jing YH; 1 Institute of Anatomy, Histology, Embryology, and Neuroscience, School of Basic Medical Sciences, Lanzhou University , Lanzhou, P.R. China .; 4 Key Laboratory of Preclinical Study for New Drugs of Gansu Province, Lanzhou University , Lanzhou, P.R. China . |
| Abstrakt: |
Liu, Xiang-Wen, Jie Yin, Qi-Sheng Ma, Chu-Chu Qi, Ji-Ying Mu, Lang Zhang, Li-Ping Gao, and Yu-Hong Jing. Role of arcuate nucleus in the regulation of feeding behavior in the process of altitude acclimatization in rats. High Alt Med Biol. 18:234-241, 2017.-Highly efficient energy utilization and metabolic homeostasis maintenance rely on neuromodulation. Altitude exposure is known to stimulate neuroendocrine systems to respond to acute hypoxia and adaptive acclimatization. However, limited data on how the adaptive regulation of the arcuate nucleus performs in the process of altitude acclimatization are available. In the present study, male Sprague Dawley rats were transported to Huashixia, Qinghai (with an altitude of 4400 m) from Xian (with an altitude of 300 m) by air; rats were consistently raised in Xian as control. Food uptake and body weight were measured consecutively after being subjected to high-altitude condition. Contents of plasma leptin and ghrelin were analyzed by the Enzyme Linked Immunosorbent Assay (ELISA) Kits. Brain coronal sections were obtained, and neuropeptide Y (NPY), proopiomelanocotin (POMC), and c-fos immunoreactivity in arcuate nucleus were observed. Arcuate nucleus was isolated from the hypothalamus, and the mRNA of NPY and POMC were measured by quantitative real-time polymerase chain reaction. Our results showed both food consumption and body weight decreased in the high plateau compared with rats raised in the low-altitude condition. Plasma leptin increased at the early stage, and ghrelin decreased at a later stage after reaching the high plateau. The peak of c-fos immunoreactivity in the arcuate nucleus was at day 3 after reaching the high plateau. The expression level of NPY increased, and POMC decreased in the arcuate nucleus at day 7 after reaching the high plateau compared with the plain control group. These results indicate that the arcuate nucleus of hypothalamus performs an important function in regulating feeding behavior during altitude acclimatization. Our study suggested that altitude acclimation is regulated by the hypothalamus that received leptin and ghrelin signals to response by its microcircuit, including NPY- and POMC-neurons in the arcuate nucleus. |
