| Autor: |
Ganaja KA, Chaplan CA, Zhang J; St. Paul's School , Concord, New Hampshire 03301, United States., Martinez NW, Martinez AW |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical chemistry [Anal Chem] 2017 May 16; Vol. 89 (10), pp. 5333-5341. Date of Electronic Publication: 2017 May 04. |
| DOI: |
10.1021/acs.analchem.6b05111 |
| Abstrakt: |
Paper microzone plates in combination with a noncontact liquid handling robot were demonstrated as tools for studying the stability of enzymes stored on paper. The effect of trehalose and SU-8 epoxy novolac resin (SU-8) on the stability of horseradish peroxidase (HRP) was studied in both a short-term experiment, where the activity of various concentrations of HRP dried on paper were measured after 1 h, and a long-term experiment, where the activity of a single concentration of HRP dried and stored on paper was monitored for 61 days. SU-8 was found to stabilize HRP up to 35 times more than trehalose in the short-term experiment for comparable concentrations of the two reagents, and a 1% SU-8 solution was found to stabilize HRP approximately 2 times more than a 34% trehalose solution in both short- and long-term experiments. The results suggest that SU-8 is a promising candidate for use as an enzyme-stabilizing reagent for paper-based diagnostic devices and that the short-term experiment could be used to quickly evaluate the capacity of various reagents for stabilizing enzymes to identify and characterize new enzyme-stabilizing reagents. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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